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Literature summary for 3.2.1.21 extracted from

  • Chuenchor, W.; Pengthaisong, S.; Robinson, R.C.; Yuvaniyama, J.; Oonanant, W.; Bevan, D.R.; Esen, A.; Chen, C.J.; Opassiri, R.; Svasti, J.; Cairns, J.R.
    Structural insights into rice BGlu1 beta-glucosidase oligosaccharide hydrolysis and transglycosylation (2008), J. Mol. Biol., 377, 1200-1215.
    View publication on PubMed
Search for more literature for 3.2.1.21

Cloned(Commentary)

Cloned (Comment) Organism
-
 Oryza sativa

Crystallization (Commentary)

Crystallization (Comment) Organism
free enzyme and its covalent intermediate with 2-deoxy-2-fluoroglucoside at 2.2 A and 1.55 A resolution, respectively. The complex with the 2-fluoroglucoside includes a glycerol molecule, which may make a nucleophilic attack on the anomeric carbon in a transglycosylation reaction. Sugars are positioned as acceptors for transglycosylation by their interactions with E176, the catalytic acid/base, and Y131. Residues I179, N190 and N245 appear to interact with the substrates Oryza sativa

Protein Variants

Protein Variants Comment Organism
I179V decrease in catalytic efficiencies Oryza sativa
L442R catalytic efficiencies similar to wild-type Oryza sativa
N190H threefold increase in catalytic efficiency towards cellobiose Oryza sativa
N245V twofold increase in catalytic efficiency towards cellobiose Oryza sativa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.139
-
 4-nitrophenyl-beta-D-glucopyranoside mutant N190H, pH 5.0, 30°C Oryza sativa
0.16
-
 cellotriose mutant N190H, pH 5.0, 30°C Oryza sativa
0.201
-
 4-nitrophenyl-beta-D-glucopyranoside mutant L442R, pH 5.0, 30°C Oryza sativa
0.201
-
 4-nitrophenyl-beta-D-glucopyranoside wild-type, pH 5.0, 30°C Oryza sativa
0.22
-
 cellotriose wild-type, pH 5.0, 30°C Oryza sativa
0.23
-
 cellotriose mutant L442R, pH 5.0, 30°C Oryza sativa
0.95
-
 4-nitrophenyl-beta-D-glucopyranoside mutant I179V, pH 5.0, 30°C Oryza sativa
1.08
-
 cellotriose mutant I179V, pH 5.0, 30°C Oryza sativa
2.1
-
 4-nitrophenyl-beta-D-glucopyranoside mutant N245V, pH 5.0, 30°C Oryza sativa
9.25
-
 cellotriose mutant N245V, pH 5.0, 30°C Oryza sativa
14.3
-
 cellobiose mutant N190H, pH 5.0, 30°C Oryza sativa
21.9
-
 cellobiose mutant L442R, pH 5.0, 30°C Oryza sativa
22
-
 cellobiose wild-type, pH 5.0, 30°C Oryza sativa
61.1
-
 cellobiose mutant I179V, pH 5.0, 30°C Oryza sativa
74.4
-
 cellobiose mutant N245V, pH 5.0, 30°C Oryza sativa

Organism

Organism UniProt Comment Textmining
Oryza sativa Q75I93
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Oryza sativa 4-nitrophenol + D-glucose
-
 ?
cellobiose + H2O
-
 Oryza sativa 2 D-glucose
-
 ?
cellotriose + H2O
-
 Oryza sativa D-glucose + cellobiose
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.12
-
 cellobiose mutant L442R, pH 5.0, 30°C Oryza sativa
1.16
-
 cellobiose wild-type, pH 5.0, 30°C Oryza sativa
2.09
-
 cellobiose mutant N190H, pH 5.0, 30°C Oryza sativa
2.35
-
 cellotriose wild-type, pH 5.0, 30°C Oryza sativa
2.58
-
 cellotriose mutant L442R, pH 5.0, 30°C Oryza sativa
3.05
-
 cellotriose mutant N190H, pH 5.0, 30°C Oryza sativa
3.3
-
 cellotriose mutant I179V, pH 5.0, 30°C Oryza sativa
3.42
-
 cellobiose mutant I179V, pH 5.0, 30°C Oryza sativa
4.57
-
 4-nitrophenyl-beta-D-glucopyranoside mutant L442R, pH 5.0, 30°C Oryza sativa
4.69
-
 4-nitrophenyl-beta-D-glucopyranoside wild-type, pH 5.0, 30°C Oryza sativa
4.7
-
 4-nitrophenyl-beta-D-glucopyranoside mutant N245V, pH 5.0, 30°C Oryza sativa
6.08
-
 4-nitrophenyl-beta-D-glucopyranoside mutant I179V, pH 5.0, 30°C Oryza sativa
6.58
-
 cellotriose mutant N245V, pH 5.0, 30°C Oryza sativa
6.76
-
 4-nitrophenyl-beta-D-glucopyranoside mutant N190H, pH 5.0, 30°C Oryza sativa
7.17
-
 cellobiose mutant N245V, pH 5.0, 30°C Oryza sativa