KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.45 | - |
4-nitrophenyl-beta-D-glucopyranoside | pH 5.0, 55°C | Sclerotinia sclerotiorum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sclerotinia sclerotiorum | - |
isolated from sunflower Helianthus annuus in Tunisia | - |
Purification (Comment) | Organism |
---|---|
native beta-glu2 60fold to homogeneity by ammonium sulfate precipitation, gel filtration, anion exchange chromatography, and chromatofocusing | Sclerotinia sclerotiorum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
584 | - |
purified beta-glu2 | Sclerotinia sclerotiorum |
Storage Stability | Organism |
---|---|
25°C, 1% SDS or urea, 1 week, 60% remaining activity | Sclerotinia sclerotiorum |
25°C, 1% Tween 20 or Triton X-100, 1 week, 90% remaining activity | Sclerotinia sclerotiorum |
90% of beta-Glu2 activity is retained when the enzyme is incubated for 6 days in the presence of 5 mg of BSA/ml or non-specific IgG | Sclerotinia sclerotiorum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Sclerotinia sclerotiorum | 4-nitrophenol + beta-D-glucose | - |
? | |
4-nitrophenyl beta-D-xylopyranoside + H2O | - |
Sclerotinia sclerotiorum | 4-nitrophenol + beta-D-xylose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
beta-glu2 | - |
Sclerotinia sclerotiorum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
assay at | Sclerotinia sclerotiorum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
purified enzyme, half-life is 85 min | Sclerotinia sclerotiorum |
65 | - |
purified enzyme, half-life is 25 min | Sclerotinia sclerotiorum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
assay at | Sclerotinia sclerotiorum |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Sclerotinia sclerotiorum | polybuffer exchanger PBE 94 TM chromatofocusing | - |
4.45 |