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Literature summary for 3.2.1.21 extracted from

  • Fleury, D.; Domaingue, P.; Gillard, C.; Touitou, R.; Mollat, P.
    Expression, purification, characterization and crystallization of a recombinant human cytosolic beta-glucosidase produced in insect cells (2007), Protein Expr. Purif., 52, 96-103.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of N-terminally His6-tagged cytosolic isozyme in Spodoptera frugiperda Sf9 cells using the baculovirus transfection system Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinnat enzyme, hanging drop vapour diffusion method, micro-seeding, the protein crystallized in 1.6 M ammonium sulfate, 100 mM HEPES, pH 7.0, within 4 weeks, X-ray diffraction structure determination and analysis Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.032
-
4-methylumbelliferyl-beta-D-glucopyranoside pH 7.0, recombinant enzyme Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Homo sapiens 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
46500
-
recombinant His-tagged enzyme, gel filtration Homo sapiens
55000
-
1 * 55000, recombinant His-tagged enzyme, SDS-PAGE, 1 * 57346, mass spectrometry Homo sapiens
57346
-
1 * 55000, recombinant His-tagged enzyme, SDS-PAGE, 1 * 57346, mass spectrometry Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant N-terminally His6-tagged cytosolic isozyme from Sf9 insect cells by nickel affinity and ion exchange chromatography to homogeneity Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.97
-
purified recombinant His-tagged enzyme, pH 5.7 Homo sapiens
2.62
-
purified recombinant His-tagged enzyme, pH 7.0 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl-beta-D-glucopyranoside + H2O
-
Homo sapiens 4-methylumbelliferol + beta-D-glucose
-
?

Subunits

Subunits Comment Organism
monomer 1 * 55000, recombinant His-tagged enzyme, SDS-PAGE, 1 * 57346, mass spectrometry Homo sapiens

Synonyms

Synonyms Comment Organism
CBG
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.7 7 assay at Homo sapiens