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Literature summary for 3.2.1.21 extracted from

  • Barbagallo, R.N.; Palmeri, R.; Fabiano, S.; Rapisarda, P.; Spagna, G.
    Characteristic of beta-glucosidase from Sicilian blood oranges in relation to anthocyanin degradation (2007), Enzyme Microb. Technol., 41, 570-575.
No PubMed abstract available
Search for more literature for 3.2.1.21

Inhibitors

Inhibitors Comment Organism Structure
D-glucose competitive inhibition Citrus sinensis
additional information no inhibition by fructose and ethanol Citrus sinensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.267
-
 4-nitrophenyl-beta-D-glucopyranoside pH 4.0, 30°C Citrus sinensis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
 x * 65000 + x * 55000, SDS-PAGE Citrus sinensis
65000
-
 x * 65000 + x * 55000, SDS-PAGE Citrus sinensis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cyanidin-3-O-beta-D-glucoside + H2O Citrus sinensis the enzyme is involved in anthocyanin metabolism, overview cyanidin + beta-D-glucose
-
 ?

Organism

Organism UniProt Comment Textmining
Citrus sinensis
-
 Sicilian sweet orange, a variety known as blood orange: Tarocco
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme partially from juice of ripe fruits by ammonium sulfate fractionation Citrus sinensis

Source Tissue

Source Tissue Comment Organism Textmining
fruit juice and pulp Citrus sinensis
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 determination of the anthocyanin content of fruits at different stages of ripening, overview Citrus sinensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Citrus sinensis 4-nitrophenol + beta-D-glucose
-
 ?
cyanidin-3-O-beta-D-glucoside + H2O
-
 Citrus sinensis cyanidin + beta-D-glucose
-
 ?
cyanidin-3-O-beta-D-glucoside + H2O the enzyme is involved in anthocyanin metabolism, overview Citrus sinensis cyanidin + beta-D-glucose
-
 ?

Subunits

Subunits Comment Organism
? x * 65000 + x * 55000, SDS-PAGE Citrus sinensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
-
 Citrus sinensis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
21 90
-
 Citrus sinensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Citrus sinensis

pH Range

pH Minimum pH Maximum Comment Organism
2.5 8
-
 Citrus sinensis

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
30.8
-
 D-glucose pH 4.0, 30°C Citrus sinensis