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Literature summary for 3.2.1.21 extracted from

  • Tsukada, T.; Igarashi, K.; Yoshida, M.; Samejima, M.
    Molecular cloning and characterization of two intracellular beta-glucosidases belonging to glycoside hydrolase family 1 from the basidiomycete Phanerochaete chrysosporium (2006), Appl. Microbiol. Biotechnol., 73, 807-814.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
isozyme BGL1A, DNA and amino acid sequence determination and analysis, transcription analysis, expression of C-terminally His-tagged enzyme in Escherichia coli Phanerodontia chrysosporium
isozyme BGL1B, DNA and amino acid sequence determination and analysis, transcription analysis, expression of C-terminally His-tagged enzyme in Escherichia coli Phanerodontia chrysosporium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.229
-
4-nitrophenyl-beta-D-glucopyranoside pH 6.5, 30°C, recombinant His-tagged isozyme BGL1A Phanerodontia chrysosporium
0.619
-
4-nitrophenyl-beta-D-glucopyranoside pH 6.5, 30°C, recombinant His-tagged isozyme BGL1A Phanerodontia chrysosporium
0.752
-
4-nitrophenyl-beta-D-xylopyranoside pH 6.5, 30°C, recombinant His-tagged isozyme BGL1A Phanerodontia chrysosporium
4.02
-
4-nitrophenyl-beta-D-galactopyranoside pH 6.5, 30°C, recombinant His-tagged isozyme BGL1A Phanerodontia chrysosporium
6.8
-
cellobiose pH 6.5, 30°C, recombinant His-tagged isozyme BGL1A Phanerodontia chrysosporium
10.2
-
4-nitrophenyl-beta-D-galactopyranoside pH 6.5, 30°C, recombinant His-tagged isozyme BGL1A Phanerodontia chrysosporium

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
53000
-
x * 53000, recombinant His-tagged isozyme BGL1A, SDS-PAGE Phanerodontia chrysosporium
60000
-
x * 60000, recombinant His-tagged isozyme BGL1B, SDS-PAGE Phanerodontia chrysosporium

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cellobiose + H2O Phanerodontia chrysosporium
-
2 beta-D-glucose
-
?
cellobiose + H2O Phanerodontia chrysosporium K-3
-
2 beta-D-glucose
-
?

Organism

Organism UniProt Comment Textmining
Phanerodontia chrysosporium Q25BW4 isozyme BGL1B; gene bgl1B, isozyme BGL1B
-
Phanerodontia chrysosporium Q25BW5 isozyme BGL1A; gene bgl1A, isozyme BGL1A
-
Phanerodontia chrysosporium K-3 Q25BW4 isozyme BGL1B; gene bgl1B, isozyme BGL1B
-
Phanerodontia chrysosporium K-3 Q25BW5 isozyme BGL1A; gene bgl1A, isozyme BGL1A
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged isozyme BGL1A from Escherichia coli by ammonium sulfate fractionation, hydrophobic interaction chromatography, and nickel affinity chromatography Phanerodontia chrysosporium
recombinant His-tagged isozyme BGL1B from Escherichia coli by ammonium sulfate fractionation, hydrophobic interaction chromatography, and nickel affinity chromatography Phanerodontia chrysosporium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl beta-D-galactopyranoside + H2O
-
Phanerodontia chrysosporium 4-nitrophenol + beta-D-galactose
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
-
Phanerodontia chrysosporium K-3 4-nitrophenol + beta-D-galactose
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
-
Phanerodontia chrysosporium 4-nitrophenol + beta-D-glucose
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
-
Phanerodontia chrysosporium K-3 4-nitrophenol + beta-D-glucose
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O low activity Phanerodontia chrysosporium 4-nitrophenol + beta-D-xylose
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O low activity Phanerodontia chrysosporium K-3 4-nitrophenol + beta-D-xylose
-
?
cellobiose + H2O
-
Phanerodontia chrysosporium 2 beta-D-glucose
-
?
cellobiose + H2O
-
Phanerodontia chrysosporium K-3 2 beta-D-glucose
-
?
additional information substrate specificity of isozyme BGL1A, overview Phanerodontia chrysosporium ?
-
?
additional information substrate specificity of isozyme BGL1B, no activity with 4-nitrophenyl-beta-D-xylopyranoside, overview Phanerodontia chrysosporium ?
-
?
additional information substrate specificity of isozyme BGL1B, no activity with 4-nitrophenyl-beta-D-xylopyranoside, overview Phanerodontia chrysosporium K-3 ?
-
?
additional information substrate specificity of isozyme BGL1A, overview Phanerodontia chrysosporium K-3 ?
-
?

Subunits

Subunits Comment Organism
? x * 53000, recombinant His-tagged isozyme BGL1A, SDS-PAGE Phanerodontia chrysosporium
? x * 60000, recombinant His-tagged isozyme BGL1B, SDS-PAGE Phanerodontia chrysosporium

Synonyms

Synonyms Comment Organism
BGL1A
-
Phanerodontia chrysosporium
BGL1B
-
Phanerodontia chrysosporium
More the enzyme belongs to the glycosyl hydrolase family 1 Phanerodontia chrysosporium

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Phanerodontia chrysosporium

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.04 1.97 4-nitrophenyl-beta-D-glucopyranoside pH 6.5, 30°C, recombinant His-tagged isozyme BGL1A Phanerodontia chrysosporium
0.04 1.97 4-nitrophenyl-beta-D-galactopyranoside pH 6.5, 30°C, recombinant His-tagged isozyme BGL1A Phanerodontia chrysosporium
0.384
-
4-nitrophenyl-beta-D-xylopyranoside pH 6.5, 30°C, recombinant His-tagged isozyme BGL1A Phanerodontia chrysosporium
0.9
-
4-nitrophenyl-beta-D-galactopyranoside pH 6.5, 30°C, recombinant His-tagged isozyme BGL1A Phanerodontia chrysosporium
9.15
-
4-nitrophenyl-beta-D-galactopyranoside pH 6.5, 30°C, recombinant His-tagged isozyme BGL1A Phanerodontia chrysosporium
9.35
-
4-nitrophenyl-beta-D-glucopyranoside pH 6.5, 30°C, recombinant His-tagged isozyme BGL1A Phanerodontia chrysosporium
20.8
-
4-nitrophenyl-beta-D-glucopyranoside pH 6.5, 30°C, recombinant His-tagged isozyme BGL1A Phanerodontia chrysosporium
67.6
-
4-nitrophenyl-beta-D-galactopyranoside pH 6.5, 30°C, recombinant His-tagged isozyme BGL1A Phanerodontia chrysosporium

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
assay at Phanerodontia chrysosporium