Cloned (Comment) | Organism |
---|---|
isozyme BGL1A, DNA and amino acid sequence determination and analysis, transcription analysis, expression of C-terminally His-tagged enzyme in Escherichia coli | Phanerodontia chrysosporium |
isozyme BGL1B, DNA and amino acid sequence determination and analysis, transcription analysis, expression of C-terminally His-tagged enzyme in Escherichia coli | Phanerodontia chrysosporium |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.229 | - |
4-nitrophenyl-beta-D-glucopyranoside | pH 6.5, 30°C, recombinant His-tagged isozyme BGL1A | Phanerodontia chrysosporium | |
0.619 | - |
4-nitrophenyl-beta-D-glucopyranoside | pH 6.5, 30°C, recombinant His-tagged isozyme BGL1A | Phanerodontia chrysosporium | |
0.752 | - |
4-nitrophenyl-beta-D-xylopyranoside | pH 6.5, 30°C, recombinant His-tagged isozyme BGL1A | Phanerodontia chrysosporium | |
4.02 | - |
4-nitrophenyl-beta-D-galactopyranoside | pH 6.5, 30°C, recombinant His-tagged isozyme BGL1A | Phanerodontia chrysosporium | |
6.8 | - |
cellobiose | pH 6.5, 30°C, recombinant His-tagged isozyme BGL1A | Phanerodontia chrysosporium | |
10.2 | - |
4-nitrophenyl-beta-D-galactopyranoside | pH 6.5, 30°C, recombinant His-tagged isozyme BGL1A | Phanerodontia chrysosporium |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
53000 | - |
x * 53000, recombinant His-tagged isozyme BGL1A, SDS-PAGE | Phanerodontia chrysosporium |
60000 | - |
x * 60000, recombinant His-tagged isozyme BGL1B, SDS-PAGE | Phanerodontia chrysosporium |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cellobiose + H2O | Phanerodontia chrysosporium | - |
2 beta-D-glucose | - |
? | |
cellobiose + H2O | Phanerodontia chrysosporium K-3 | - |
2 beta-D-glucose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Phanerodontia chrysosporium | Q25BW4 | isozyme BGL1B; gene bgl1B, isozyme BGL1B | - |
Phanerodontia chrysosporium | Q25BW5 | isozyme BGL1A; gene bgl1A, isozyme BGL1A | - |
Phanerodontia chrysosporium K-3 | Q25BW4 | isozyme BGL1B; gene bgl1B, isozyme BGL1B | - |
Phanerodontia chrysosporium K-3 | Q25BW5 | isozyme BGL1A; gene bgl1A, isozyme BGL1A | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged isozyme BGL1A from Escherichia coli by ammonium sulfate fractionation, hydrophobic interaction chromatography, and nickel affinity chromatography | Phanerodontia chrysosporium |
recombinant His-tagged isozyme BGL1B from Escherichia coli by ammonium sulfate fractionation, hydrophobic interaction chromatography, and nickel affinity chromatography | Phanerodontia chrysosporium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl beta-D-galactopyranoside + H2O | - |
Phanerodontia chrysosporium | 4-nitrophenol + beta-D-galactose | - |
? | |
4-nitrophenyl beta-D-galactopyranoside + H2O | - |
Phanerodontia chrysosporium K-3 | 4-nitrophenol + beta-D-galactose | - |
? | |
4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Phanerodontia chrysosporium | 4-nitrophenol + beta-D-glucose | - |
? | |
4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Phanerodontia chrysosporium K-3 | 4-nitrophenol + beta-D-glucose | - |
? | |
4-nitrophenyl beta-D-xylopyranoside + H2O | low activity | Phanerodontia chrysosporium | 4-nitrophenol + beta-D-xylose | - |
? | |
4-nitrophenyl beta-D-xylopyranoside + H2O | low activity | Phanerodontia chrysosporium K-3 | 4-nitrophenol + beta-D-xylose | - |
? | |
cellobiose + H2O | - |
Phanerodontia chrysosporium | 2 beta-D-glucose | - |
? | |
cellobiose + H2O | - |
Phanerodontia chrysosporium K-3 | 2 beta-D-glucose | - |
? | |
additional information | substrate specificity of isozyme BGL1A, overview | Phanerodontia chrysosporium | ? | - |
? | |
additional information | substrate specificity of isozyme BGL1B, no activity with 4-nitrophenyl-beta-D-xylopyranoside, overview | Phanerodontia chrysosporium | ? | - |
? | |
additional information | substrate specificity of isozyme BGL1B, no activity with 4-nitrophenyl-beta-D-xylopyranoside, overview | Phanerodontia chrysosporium K-3 | ? | - |
? | |
additional information | substrate specificity of isozyme BGL1A, overview | Phanerodontia chrysosporium K-3 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 53000, recombinant His-tagged isozyme BGL1A, SDS-PAGE | Phanerodontia chrysosporium |
? | x * 60000, recombinant His-tagged isozyme BGL1B, SDS-PAGE | Phanerodontia chrysosporium |
Synonyms | Comment | Organism |
---|---|---|
BGL1A | - |
Phanerodontia chrysosporium |
BGL1B | - |
Phanerodontia chrysosporium |
More | the enzyme belongs to the glycosyl hydrolase family 1 | Phanerodontia chrysosporium |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Phanerodontia chrysosporium |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | 1.97 | 4-nitrophenyl-beta-D-glucopyranoside | pH 6.5, 30°C, recombinant His-tagged isozyme BGL1A | Phanerodontia chrysosporium | |
0.04 | 1.97 | 4-nitrophenyl-beta-D-galactopyranoside | pH 6.5, 30°C, recombinant His-tagged isozyme BGL1A | Phanerodontia chrysosporium | |
0.384 | - |
4-nitrophenyl-beta-D-xylopyranoside | pH 6.5, 30°C, recombinant His-tagged isozyme BGL1A | Phanerodontia chrysosporium | |
0.9 | - |
4-nitrophenyl-beta-D-galactopyranoside | pH 6.5, 30°C, recombinant His-tagged isozyme BGL1A | Phanerodontia chrysosporium | |
9.15 | - |
4-nitrophenyl-beta-D-galactopyranoside | pH 6.5, 30°C, recombinant His-tagged isozyme BGL1A | Phanerodontia chrysosporium | |
9.35 | - |
4-nitrophenyl-beta-D-glucopyranoside | pH 6.5, 30°C, recombinant His-tagged isozyme BGL1A | Phanerodontia chrysosporium | |
20.8 | - |
4-nitrophenyl-beta-D-glucopyranoside | pH 6.5, 30°C, recombinant His-tagged isozyme BGL1A | Phanerodontia chrysosporium | |
67.6 | - |
4-nitrophenyl-beta-D-galactopyranoside | pH 6.5, 30°C, recombinant His-tagged isozyme BGL1A | Phanerodontia chrysosporium |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
assay at | Phanerodontia chrysosporium |