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Literature summary for 3.2.1.21 extracted from

  • Ni, J.; Tokuda, G.; Takehara, M.; Watanabe, H.
    Heterologous expression and enzymatic characterization of beta-glucosidase from the drywood-eating termite, Neotermes koshunensis (2007), Appl. Entomol. Zool., 42, 457-463.
No PubMed abstract available
Search for more literature for 3.2.1.21

Cloned(Commentary)

Cloned (Comment) Organism
overexpression of His-tagged enzyme in Escherichia coli strain JM109 Neotermes koshunensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.8
-
 cellobiose pH 5.5, 37°C, recombinant His-tagged enzyme Neotermes koshunensis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
 x * 60000, recombinant His-tagged enzyme, SDS-PAGE Neotermes koshunensis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cellobiose + H2O Neotermes koshunensis
-
 2 beta-D-glucose
-
 ?

Organism

Organism UniProt Comment Textmining
Neotermes koshunensis Q8T0W7 drywood-eating termite
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme 6.4fold from Escherichia coli to homogeneity by nickel affinity chromatography Neotermes koshunensis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
156.7
-
 purified recombinant His-tagged enzyme Neotermes koshunensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl beta-D-fucopyranoside + H2O high activity Neotermes koshunensis 4-nitrophenol + beta-D-fucose
-
 ?
4-nitrophenyl beta-D-galactopyranoside + H2O low activity Neotermes koshunensis 4-nitrophenol + beta-D-galactose
-
 ?
4-nitrophenyl beta-D-glucopyranoside + H2O low activity Neotermes koshunensis 4-nitrophenol + beta-D-glucose
-
 ?
cellobiose + H2O
-
 Neotermes koshunensis 2 beta-D-glucose
-
 ?
cellobiose + H2O high activity Neotermes koshunensis 2 beta-D-glucose
-
 ?
cellotetraose + 3 H2O low activity Neotermes koshunensis 4 beta-D-glucose
-
 ?
cellotriose + H2O low activity Neotermes koshunensis 3 D-glucose
-
 ?
laminaribiose + H2O high activity Neotermes koshunensis 2 beta-D-glucose
-
 ?
laminarin + H2O low activity Neotermes koshunensis beta-D-glucose
-
 ?
additional information substrate specificity of the recombinant His-tagged enzyme, gentiobiose, lactose, and salicin are poor substrates, melibiose, palatinose, sophorose, and cellulose are no substrates, overview Neotermes koshunensis ?
-
 ?

Subunits

Subunits Comment Organism
? x * 60000, recombinant His-tagged enzyme, SDS-PAGE Neotermes koshunensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
 recombinant His-tagged enzyme Neotermes koshunensis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
10 50 activity range Neotermes koshunensis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 purified recombinant His-tagged enzyme, 30 min, 80% remianing activity Neotermes koshunensis
45
-
 purified recombinant His-tagged enzyme, stable up to Neotermes koshunensis
50
-
 purified recombinant His-tagged enzyme, inactivation Neotermes koshunensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
 recombinant His-tagged enzyme Neotermes koshunensis

pH Range

pH Minimum pH Maximum Comment Organism
4 8.5 activity range Neotermes koshunensis