Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ethanol | activation depends on the yeast strain, e.g. strain Y8 shows 5fold increased activity at 14% ethanol in wine solution, overview | Hanseniaspora uvarum | |
ethanol | strain AL41 shows increased activity at 14% ethanol in wine solution | Saccharomyces cerevisiae |
Application | Comment | Organism |
---|---|---|
nutrition | the enzyme is useful in enological applications during the final stages of alcoholic fermentations in addition to yeast strains, overview | Aspergillus niger |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
D-fructose | - |
Aspergillus niger | |
D-glucose | - |
Aspergillus niger | |
ethanol | inhibition at ethanol concentration below 10% | Aspergillus niger | |
ethanol | inhibition at concentration below 10%, inhibition of 40-60% at about 12-14% ethanol in wine solution of strain CBS314, depends on the yeast strain, overview | Hanseniaspora uvarum | |
ethanol | inhibition at concentration above 10% | Saccharomyces cerevisiae | |
ethanol | inhibition at concentration above 10% | Wickerhamomyces anomalus | |
additional information | no inhibition by D-glucose and D-fructose | Hanseniaspora uvarum | |
additional information | no inhibition by D-glucose and D-fructose | Saccharomyces cerevisiae | |
additional information | no inhibition by D-glucose and D-fructose | Wickerhamomyces anomalus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | the enzyme does not follow Michaelis-Menten kinetics | Aspergillus niger | |
0.47 | - |
4-nitrophenyl beta-D-glucoside | pH 3.5, 20°C | Wickerhamomyces anomalus | |
0.61 | 0.71 | 4-nitrophenyl beta-D-glucoside | enzymes from several different strains | Hanseniaspora uvarum | |
2.5 | 3 | 4-nitrophenyl beta-D-glucoside | pH 3.5, 20°C | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus niger | - |
- |
- |
Hanseniaspora uvarum | - |
diverse strains isolated from sicilian wines and musts, e.g. strains CBS314 and Y8 with high enzyme activity | - |
Saccharomyces cerevisiae | - |
isolated from sicilian wine and must | - |
Saccharomyces cerevisiae AL41 | - |
isolated from sicilian wine and must | - |
Wickerhamomyces anomalus | - |
isolated from sicilian wine and must | - |
Wickerhamomyces anomalus AL112 | - |
isolated from sicilian wine and must | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
commercial preparation | purified enzyme | Aspergillus niger | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
comparison of enzyme activity in isolated Saccharomyces cerevisiae strains from wines and musts with activity of the purified enzyme from commercial preparation of Aspergillus niger | Aspergillus niger |
additional information | - |
comparison of enzyme activity in isolated strains from wines and musts with activity of a commercial preparation from Aspergillus niger | Saccharomyces cerevisiae |
additional information | - |
comparison of enzyme activity in isolated strains from wines and musts with activity of a commercial preparation from Aspergillus niger | Hanseniaspora uvarum |
Storage Stability | Organism |
---|---|
20°C, enzyme in wine solution, stable for 35-45 days | Saccharomyces cerevisiae |
20°C, enzyme in wine solution, stable for at least 40 days | Hanseniaspora uvarum |
20°C, enzyme in wine solution, stable for over 45 days | Wickerhamomyces anomalus |
20°C, purified enzyme in wine solution, stable for 12 days | Aspergillus niger |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Saccharomyces cerevisiae | 4-nitrophenol + D-glucopyranose | - |
? | |
4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Wickerhamomyces anomalus | 4-nitrophenol + D-glucopyranose | - |
? | |
4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Wickerhamomyces anomalus AL112 | 4-nitrophenol + D-glucopyranose | - |
? | |
4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Saccharomyces cerevisiae AL41 | 4-nitrophenol + D-glucopyranose | - |
? | |
4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Wickerhamomyces anomalus | 4-nitrophenol + beta-D-glucose | - |
? | |
4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Hanseniaspora uvarum | 4-nitrophenol + beta-D-glucose | - |
? | |
4-nitrophenyl beta-D-glucopyranoside + H2O | hydrolysis of beta-linkages | Saccharomyces cerevisiae | 4-nitrophenol + beta-D-glucose | - |
? | |
4-nitrophenyl beta-D-glucopyranoside + H2O | hydrolysis of beta-linkages | Aspergillus niger | 4-nitrophenol + beta-D-glucose | - |
? | |
4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Wickerhamomyces anomalus AL112 | 4-nitrophenol + beta-D-glucose | - |
? | |
4-nitrophenyl beta-D-glucopyranoside + H2O | hydrolysis of beta-linkages | Saccharomyces cerevisiae AL41 | 4-nitrophenol + beta-D-glucose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
betaG | - |
Saccharomyces cerevisiae |
betaG | - |
Aspergillus niger |
betaG | - |
Wickerhamomyces anomalus |
betaG | - |
Hanseniaspora uvarum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
- |
Saccharomyces cerevisiae |
20 | - |
- |
Wickerhamomyces anomalus |
20 | - |
strain Y8 | Hanseniaspora uvarum |
25 | - |
strain CBS314 | Hanseniaspora uvarum |
62 | - |
- |
Aspergillus niger |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | 90 | - |
Aspergillus niger |
10 | 35 | - |
Saccharomyces cerevisiae |
10 | 35 | - |
Wickerhamomyces anomalus |
10 | 35 | - |
Hanseniaspora uvarum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
3.5 | - |
- |
Aspergillus niger |
4 | 4.5 | - |
Saccharomyces cerevisiae |
5 | - |
- |
Hanseniaspora uvarum |
5.5 | - |
- |
Wickerhamomyces anomalus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 7 | - |
Saccharomyces cerevisiae |
3 | 7 | - |
Wickerhamomyces anomalus |
4 | 6.5 | strain CBS314 | Hanseniaspora uvarum |
4 | 5 | strain Y8 | Hanseniaspora uvarum |