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Literature summary for 3.2.1.21 extracted from
- Purification, crystallization and preliminary X-ray analysis of a hexameric beta-glucosidase from wheat (2005), Acta Crystallogr. Sect. F, 61, 864-866.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression of His-tagged Glu1b in Escherichia coli as soluble protein | Triticum aestivum |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| purified recombinant enzyme complexed with a substrate aglycone, 20°C, hanging drop vapour diffusion method, 0.001 ml of 3.7 mg/ml protein in solution mixed with 0.001 ml reservoir solution containing 10 mM HEPES, pH 7.2, 1 M LiSO4, and 150 mM NaCl, X-ray diffraction structure determination and preliminary analysis at 1.7 A resolution, molecular replacement | Triticum aestivum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Triticum aestivum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged Glu1b from Escherichia coli by nickel affinity chelating chromatography, ultrafiltration, and gel filtration | Triticum aestivum |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| hexamer | active form | Triticum aestivum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Glu1b | - |
Triticum aestivum |
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