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Literature summary for 3.2.1.21 extracted from

  • Hsieh, M.C.; Graham, T.L.
    Partial purification and characterization of a soybean beta-glucosidase with high specific activity towards isoflavone conjugates (2001), Phytochemistry, 58, 995-1005.
    View publication on PubMed
Search for more literature for 3.2.1.21

Inhibitors

Inhibitors Comment Organism Structure
Ag+ 1 mM, 65% inhibition, 10 mM, complete inhibition, 50% inhibition at 14 mM in the presence of 250 mM beta-mercaptoethanol Glycine max
beta-mercaptoethanol 1.1 M, 50% inhibition Glycine max
glucono-delta-lactone IC50: 24 mM Glycine max
Hg2+ 1 mM, 38% inhibition, 10 mM, 77% inhibition Glycine max

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.054
-
 genistein-7-O-glucosyl-6''-O-malonate 40°C, pH 5.0 Glycine max
0.091
-
 daidzein-7-O-glucoside 40°C, pH 5.0 Glycine max
0.12
-
 daidzein-7-O-glucosyl-6''-O-malonate 40°C, pH 5.0 Glycine max
0.23
-
 4-methylumbelliferyl-beta-D-glucopyranoside 40°C, pH 5.0 Glycine max
0.33
-
 genistein-7-O-glucoside 40°C, pH 5.0 Glycine max
0.84
-
 p-nitrophenyl-alpha-D-galactopyranoside 40°C, pH 5.0 Glycine max
1.3
-
 p-nitrophenyl-beta-D-glucopyranoside 40°C, pH 5.0 Glycine max
1.4
-
 o-nitrophenyl-beta-D-glucopyranoside 40°C, pH 5.0 Glycine max
3.5
-
 Salicin 40°C, pH 5.0 Glycine max
6.1
-
 arbutin 40°C, pH 5.0 Glycine max
8.8
-
 esculin 40°C, pH 5.0 Glycine max
9.9
-
 p-nitrophenyl-alpha-D-mannopyranoside 40°C, pH 5.0 Glycine max
13
-
 p-nitrophenyl-beta-D-galactopyranoside 40°C, pH 5.0 Glycine max

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
80000
-
 2 * 80000, SDS-PAGE Glycine max
165000
-
 native-PAGE Glycine max

Organism

Organism UniProt Comment Textmining
Glycine max
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate, DEAE-Sephadex, CM-Sephadex Glycine max

Source Tissue

Source Tissue Comment Organism Textmining
root
-
 Glycine max
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1340
-
-
 Glycine max

Storage Stability

Storage Stability Organism
-20°C, 10% glycerol, at least six months, no loss of activity Glycine max

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl-beta-D-glucopyranoside + H2O
-
 Glycine max 4-methylumbelliferol + beta-D-glucose
-
 ?
arbutin + H2O
-
 Glycine max 4-hydroxyphenol + beta-D-glucose
-
 ?
daidzein 7'-O-(6''-O-beta-D-malonyl-beta-D-glucoside) + H2O
-
 Glycine max daidzein + 6-O-malonyl-beta-D-glucopyranose
-
 ?
daidzein 7-O-(6-O-malonyl-beta-D-glucoside) + H2O
-
 Glycine max daidzein + 6-O-malonyl-beta-D-glucopyranose
-
 ?
daidzein 7-O-beta-D-glucopyranoside + H2O
-
 Glycine max daidzein + beta-D-glucose
-
 ?
daidzein-7-O-glucosyl-6''-O-malonate + H2O
-
 Glycine max daidzein + 6-O-malonyl-beta-D-glucopyranose
-
 ?
esculin + H2O
-
 Glycine max beta-D-glucose + esculetin
-
 ?
genistein-7-O-glucoside + H2O
-
 Glycine max genistein + beta-D-glucose
-
 ?
genistein-7-O-glucosyl-6''-O-malonate + H2O
-
 Glycine max genistein + beta-D-glucose-6-O-malonate
-
 ?
o-nitrophenyl-beta-D-glucopyranoside + H2O
-
 Glycine max o-nitrophenol + beta-D-glucose
-
 ?
p-nitrophenyl-alpha-D-galactopyranoside + H2O
-
 Glycine max p-nitrophenol + alpha-D-galactose
-
 ?
p-nitrophenyl-alpha-D-mannopyranoside + H2O
-
 Glycine max p-nitrophenol + alpha-D-mannose
-
 ?
p-nitrophenyl-beta-D-galactopyranoside + H2O
-
 Glycine max p-nitrophenol + beta-D-galactose
-
 ?
p-nitrophenyl-beta-D-glucopyranoside + H2O
-
 Glycine max p-nitrophenol + beta-D-glucose
-
 ?
salicin + H2O
-
 Glycine max beta-D-glucose + salicyl alcohol
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 80000, SDS-PAGE Glycine max

Synonyms

Synonyms Comment Organism
ICHG
-
 Glycine max

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Glycine max

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 13% loss of activity after 45 min Glycine max

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.02
-
 esculin 40°C, pH 5.0 Glycine max
0.05
-
 4-methylumbelliferyl-beta-D-glucopyranoside 40°C, pH 5.0 Glycine max
0.1
-
 p-nitrophenyl-alpha-D-mannopyranoside 40°C, pH 5.0 Glycine max
0.11
-
 p-nitrophenyl-beta-D-glucopyranoside 40°C, pH 5.0 Glycine max
0.15
-
 arbutin 40°C, pH 5.0 Glycine max
0.21
-
 Salicin 40°C, pH 5.0 Glycine max
0.49
-
 p-nitrophenyl-alpha-D-galactopyranoside 40°C, pH 5.0 Glycine max
0.93
-
 p-nitrophenyl-beta-D-galactopyranoside 40°C, pH 5.0 Glycine max
1.4
-
 o-nitrophenyl-beta-D-glucopyranoside 40°C, pH 5.0 Glycine max
3.07
-
 daidzein-7-O-glucoside 40°C, pH 5.0 Glycine max
4.34
-
 daidzein 7'-O-(6''-O-beta-D-malonyl-beta-D-glucoside) 40°C, pH 5.0 Glycine max
4.38
-
 genistein-7-O-glucosyl-6''-O-malonate 40°C, pH 5.0 Glycine max
4.45
-
 genistein-7-O-glucoside 40°C, pH 5.0 Glycine max

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 at 30°C Glycine max

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
24
-
 IC50: 24 mM Glycine max glucono-delta-lactone