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Literature summary for 3.2.1.21 extracted from

  • Venturi, L.L.; Polizeli Mde, L.; Terenzi, H.F.; Furriel Rdos, P.; Jorge, J.A.
    Extracellular beta-D-glucosidase from Chaetomium thermophilum var. coprophilum: production, purification and some biochemical properties (2002), J. Basic Microbiol., 42, 55-66.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Ag2+ 1 mM, complete inhibition Thermochaetoides thermophila
cellobiose competitive vs. p-nitrophenyl-beta-D-glucopyranoside Thermochaetoides thermophila
Hg2+ 1 mM, complete inhibition Thermochaetoides thermophila

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.76
-
p-nitrophenyl-beta-D-glucopyranoside
-
Thermochaetoides thermophila
3.13
-
cellobiose
-
Thermochaetoides thermophila

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Thermochaetoides thermophila
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
gel filtration Thermochaetoides thermophila
43000
-
1 * 43000, SDS-PAGE Thermochaetoides thermophila

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cellobiose + H2O Thermochaetoides thermophila
-
2 beta-D-glucose
-
?

Organism

Organism UniProt Comment Textmining
Thermochaetoides thermophila
-
var. coprophilum
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein heavily glycosylated Thermochaetoides thermophila

Purification (Commentary)

Purification (Comment) Organism
acetone precipitation, DEAE-cellulose Thermochaetoides thermophila

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6-bromo-2-naphthyl-beta-D-glucopyranoside + H2O
-
Thermochaetoides thermophila 6-bromo-2-naphthol + beta-D-glucose
-
?
cellobiose + H2O
-
Thermochaetoides thermophila 2 beta-D-glucose
-
?
cellobiose + H2O 0.5% of activity with p-nitrophenyl-beta-D-glucopyranoside Thermochaetoides thermophila 2 beta-D-glucose
-
?
p-nitrophenyl-beta-D-glucopyranoside + H2O
-
Thermochaetoides thermophila p-nitrophenol + beta-D-glucose
-
?

Subunits

Subunits Comment Organism
monomer 1 * 43000, SDS-PAGE Thermochaetoides thermophila

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
-
Thermochaetoides thermophila

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
35 80 approx. 50% of maximal activity at 50°C, 10% of maximal activity at 80°C Thermochaetoides thermophila

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50 60 no loss of activity after 2 h, deglycosylated beta-glucosidase, 50% loss of activity after 18 min Thermochaetoides thermophila
70
-
50% loss of activity afer 75 min Thermochaetoides thermophila

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
Thermochaetoides thermophila

pH Range

pH Minimum pH Maximum Comment Organism
3.5 8 approx. 16% of maximal activity at pH 4.5 and pH 6.5, respectively Thermochaetoides thermophila

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.8
-
cellobiose
-
Thermochaetoides thermophila

pI Value

Organism Comment pI Value Maximum pI Value
Thermochaetoides thermophila isoelectric focusing
-
7