Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag2+ | 1 mM, complete inhibition | Thermochaetoides thermophila | |
cellobiose | competitive vs. p-nitrophenyl-beta-D-glucopyranoside | Thermochaetoides thermophila | |
Hg2+ | 1 mM, complete inhibition | Thermochaetoides thermophila |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.76 | - |
p-nitrophenyl-beta-D-glucopyranoside | - |
Thermochaetoides thermophila | |
3.13 | - |
cellobiose | - |
Thermochaetoides thermophila |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Thermochaetoides thermophila | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
gel filtration | Thermochaetoides thermophila |
43000 | - |
1 * 43000, SDS-PAGE | Thermochaetoides thermophila |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cellobiose + H2O | Thermochaetoides thermophila | - |
2 beta-D-glucose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermochaetoides thermophila | - |
var. coprophilum | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | heavily glycosylated | Thermochaetoides thermophila |
Purification (Comment) | Organism |
---|---|
acetone precipitation, DEAE-cellulose | Thermochaetoides thermophila |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
6-bromo-2-naphthyl-beta-D-glucopyranoside + H2O | - |
Thermochaetoides thermophila | 6-bromo-2-naphthol + beta-D-glucose | - |
? | |
cellobiose + H2O | - |
Thermochaetoides thermophila | 2 beta-D-glucose | - |
? | |
cellobiose + H2O | 0.5% of activity with p-nitrophenyl-beta-D-glucopyranoside | Thermochaetoides thermophila | 2 beta-D-glucose | - |
? | |
p-nitrophenyl-beta-D-glucopyranoside + H2O | - |
Thermochaetoides thermophila | p-nitrophenol + beta-D-glucose | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 43000, SDS-PAGE | Thermochaetoides thermophila |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
- |
Thermochaetoides thermophila |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | 80 | approx. 50% of maximal activity at 50°C, 10% of maximal activity at 80°C | Thermochaetoides thermophila |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 60 | no loss of activity after 2 h, deglycosylated beta-glucosidase, 50% loss of activity after 18 min | Thermochaetoides thermophila |
70 | - |
50% loss of activity afer 75 min | Thermochaetoides thermophila |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
- |
Thermochaetoides thermophila |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3.5 | 8 | approx. 16% of maximal activity at pH 4.5 and pH 6.5, respectively | Thermochaetoides thermophila |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
2.8 | - |
cellobiose | - |
Thermochaetoides thermophila |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Thermochaetoides thermophila | isoelectric focusing | - |
7 |