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Literature summary for 3.2.1.21 extracted from

  • Yang, L.; Ning, Z.S.; Shi, C.Z.; Chang, Z.Y.; Huan, L.Y.
    Purification and characterization of an isoflavone-conjugates-hydrolyzing beta-glucosidase from endophytic bacterium (2004), J. Agric. Food Chem., 52, 1940-1944.
    View publication on PubMed
Search for more literature for 3.2.1.21

Activating Compound

Activating Compound Comment Organism Structure
beta-mercaptoethanol 1 mM, slight activation Pseudomonas sp.
dithioerythritol 1 mM, slight activation Pseudomonas sp.
dithiothreitol 1 mM, slight activation Pseudomonas sp.

Inhibitors

Inhibitors Comment Organism Structure
AgNO3 1 mM, 88% inhibition Pseudomonas sp.
D-glucose
-
 Pseudomonas sp.
glucono-delta-lactone
-
 Pseudomonas sp.
HgCl2 1 mM, 94% inhibition Pseudomonas sp.
iodoacetic acid 1 mM, 92% inhibition Pseudomonas sp.
N-ethylmaleimide 1 mM, complete inhibition Pseudomonas sp.
p-chloromercuribenzoate 1 mM, complete inhibition Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32000
-
 gel filtration Pseudomonas sp.
33000
-
 1 * 33000, SDS-PAGE Pseudomonas sp.

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
-
-
Pseudomonas sp. ZD-8
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate, Sephadex G-100, DEAE-Sepharose,CL-6B, DEAE-Sephacel Pseudomonas sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1485
-
-
 Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
apigenin-7-beta-D-glucopyranoside + H2O
-
 Pseudomonas sp. apigenin + beta-D-glucose
-
 ?
apigenin-7-beta-D-glucopyranoside + H2O
-
 Pseudomonas sp. ZD-8 apigenin + beta-D-glucose
-
 ?
arbutin + H2O
-
 Pseudomonas sp. beta-D-glucose + 4-hydroxyphenol
-
 ?
arbutin + H2O
-
 Pseudomonas sp. ZD-8 beta-D-glucose + 4-hydroxyphenol
-
 ?
daidzein 7-O-(6-O-malonyl-beta-D-glucoside) + H2O
-
 Pseudomonas sp. daidzein + 6-O-malonyl-beta-D-glucopyranose
-
 ?
daidzein 7-O-(6-O-malonyl-beta-D-glucoside) + H2O
-
 Pseudomonas sp. ZD-8 daidzein + 6-O-malonyl-beta-D-glucopyranose
-
 ?
daidzein-7-O-beta-D-glucopyranoside + H2O
-
 Pseudomonas sp. daidzein + beta-D-glucose
-
 ?
daidzein-7-O-beta-D-glucopyranoside + H2O
-
 Pseudomonas sp. ZD-8 daidzein + beta-D-glucose
-
 ?
esculin + H2O
-
 Pseudomonas sp. beta-D-glucose + esculetin
-
 ?
esculin + H2O
-
 Pseudomonas sp. ZD-8 beta-D-glucose + esculetin
-
 ?
genistein-7-O-beta-D-glucopyranoside + H2O
-
 Pseudomonas sp. genistein + beta-D-glucose
-
 ?
genistein-7-O-beta-D-glucosyl-6''-O-malonate + H2O
-
 Pseudomonas sp. genistein + 6-O-malonyl-beta-D-glucose
-
 ?
p-nitrophenyl-beta-D-galactopyranoside + H2O
-
 Pseudomonas sp. p-nitrophenol + beta-D-galactose
-
 ?
p-nitrophenyl-beta-D-glucopyranoside + H2O
-
 Pseudomonas sp. p-nitrophenol + beta-D-glucose
-
 ?
p-nitrophenyl-beta-D-mannopyranoside + H2O
-
 Pseudomonas sp. p-nitrophenol + beta-D-mannose
-
 ?
salicin + H2O
-
 Pseudomonas sp. beta-D-glucose + salicylic alcohol
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 33000, SDS-PAGE Pseudomonas sp.

Synonyms

Synonyms Comment Organism
ICHG
-
 Pseudomonas sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Pseudomonas sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 70 approx. 60% of maximal activity at 20°C, approx. 10% of maximal activity at 65% Pseudomonas sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
 no loss of activity after 30 min Pseudomonas sp.
40
-
 approx. 20% loss of activity after 30 min Pseudomonas sp.
50
-
 approx. 56% loss of activity after 30 min Pseudomonas sp.
60
-
 approx. 95% loss of activity after 30 min Pseudomonas sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.32
-
 p-nitrophenyl-beta-D-mannopyranoside 40°C, pH 6.0 Pseudomonas sp.
0.62
-
 p-nitrophenyl-beta-D-galactopyranoside 40°C, pH 6.0 Pseudomonas sp.
0.87
-
 p-nitrophenyl-beta-D-glucopyranoside 40°C, pH 6.0 Pseudomonas sp.
1.06
-
 arbutin 40°C, pH 6.0 Pseudomonas sp.
1.32
-
 Salicin 40°C, pH 6.0 Pseudomonas sp.
1.57
-
 esculin 40°C, pH 6.0 Pseudomonas sp.
2.39
-
 daidzein 7-O-(6-O-malonyl-beta-D-glucoside) 40°C, pH 6.0 Pseudomonas sp.
2.68
-
 genistein-7-beta-D-glucosyl-6''-O-malonate 40°C, pH 6.0 Pseudomonas sp.
3.92
-
 apigenin-7-O-beta-D-glucopyranoside 40°C, pH 6.0 Pseudomonas sp.
5.12
-
 daidzein-7-beta-D-glucopyranoside 40°C, pH 6.0 Pseudomonas sp.
5.31
-
 genistein-7-beta-D-glucopyranoside 40°C, pH 6.0 Pseudomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Pseudomonas sp.

pH Range

pH Minimum pH Maximum Comment Organism
3.5 8.5 approx. 60% of maxim activity at pH 4.0, approx. 70% of maximal activity at pH 8.0 Pseudomonas sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
3.5 8.5 approx. 30% loss of activity after 2 h at pH 3.5 and 30°C, approx. 20% loss of activity after 2 h at pH 8.5 and 30°C Pseudomonas sp.

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
23
-
 glucono-delta-lactone 40°C, pH 6.0, vs. genistein-7-O-beta-D-glucopyranoside Pseudomonas sp.
84
-
 D-glucose 40°C, pH 6.0, vs. genistein-7-O-beta-D-glucopyranoside Pseudomonas sp.

pI Value

Organism Comment pI Value Maximum pI Value
Pseudomonas sp. isoelectric focusing
-
 4.9