Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | 500 mM, 2fold activation, inhibition above | Vanilla planifolia |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,4-dithiothreitol | 1.5-2 M, complete inhibition | Vanilla planifolia | |
1-deoxynojirimycin | - |
Vanilla planifolia | |
2-mercaptoethanol | 2 M, 95% inhibition | Vanilla planifolia | |
Ag+ | 1 mM, 81% inhibition | Vanilla planifolia | |
Co2+ | 1 mM, 53% inhibition | Vanilla planifolia | |
Cu2+ | 1 mM, 96% inhibition | Vanilla planifolia | |
glucono-delta-lactone | - |
Vanilla planifolia | |
Hg2+ | 0.06 mM, 97% inhibition | Vanilla planifolia | |
Mn2+ | 1 mM, 66% inhibition | Vanilla planifolia | |
additional information | not inhibited by D-glucose up to 2 M | Vanilla planifolia | |
Ni2+ | 1 mM, 70% inhibition | Vanilla planifolia | |
Zn2+ | 1 mM, 93% inhibition | Vanilla planifolia |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.1 | - |
p-nitrophenyl-beta-D-glucopyranoside | 40°C, pH 7.0 | Vanilla planifolia | |
20 | - |
glucovanillin | 40°C, pH 7.0 | Vanilla planifolia |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
4 * 50000, SDS-PAGE | Vanilla planifolia |
201000 | - |
gel filtration | Vanilla planifolia |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
glucovanillin + H2O | Vanilla planifolia | - |
vanillin + beta-D-glucose | - |
? | |
glucovanillin + H2O | Vanilla planifolia Andrews | - |
vanillin + beta-D-glucose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vanilla planifolia | - |
Andrews | - |
Vanilla planifolia Andrews | - |
Andrews | - |
Purification (Comment) | Organism |
---|---|
DEAE-Sepharose, Phenyl-Sepharose, Sephacryl S-300 HR | Vanilla planifolia |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
177.6 | - |
- |
Vanilla planifolia |
Storage Stability | Organism |
---|---|
4°C, 20 mM Tris-HCl, pH 8.0, at least 6 months, no loss of activity | Vanilla planifolia |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
esculin + H2O | 92% of activity with p-nitrophenyl-beta-D-glucopyranoside | Vanilla planifolia | beta-D-glucose + esculetin | - |
? | |
esculin + H2O | 92% of activity with p-nitrophenyl-beta-D-glucopyranoside | Vanilla planifolia Andrews | beta-D-glucose + esculetin | - |
? | |
glucovanillin + H2O | - |
Vanilla planifolia | vanillin + beta-D-glucose | - |
? | |
glucovanillin + H2O | 22% of activity with p-nitrophenyl-beta-D-glucopyranoside | Vanilla planifolia | vanillin + beta-D-glucose | - |
? | |
glucovanillin + H2O | - |
Vanilla planifolia Andrews | vanillin + beta-D-glucose | - |
? | |
glucovanillin + H2O | 22% of activity with p-nitrophenyl-beta-D-glucopyranoside | Vanilla planifolia Andrews | vanillin + beta-D-glucose | - |
? | |
p-nitrophenyl-beta-D-fucopyranoside + H2O | 215% of activity with p-nitrophenyl-beta-D-glucopyranoside | Vanilla planifolia | p-nitrophenol + beta-D-fucose | - |
? | |
p-nitrophenyl-beta-D-fucopyranoside + H2O | 215% of activity with p-nitrophenyl-beta-D-glucopyranoside | Vanilla planifolia Andrews | p-nitrophenol + beta-D-fucose | - |
? | |
p-nitrophenyl-beta-D-galactopyranoside + H2O | 61% of activity with p-nitrophenyl-beta-D-glucopyranoside | Vanilla planifolia | p-nitrophenol + beta-D-galactose | - |
? | |
p-nitrophenyl-beta-D-glucopyranoside + H2O | - |
Vanilla planifolia | p-nitrophenol + beta-D-glucose | - |
? | |
p-nitrophenyl-beta-D-xylopyranoside + H2O | 5% of activity with p-nitrophenyl-beta-D-glucopyranoside | Vanilla planifolia | p-nitrophenol + beta-D-xylose | - |
? | |
prunasin + H2O | 300% of activity with p-nitrophenyl-beta-D-glucopyranoside | Vanilla planifolia | D-mandelonitrile + beta-D-glucose | - |
? | |
prunasin + H2O | 300% of activity with p-nitrophenyl-beta-D-glucopyranoside | Vanilla planifolia Andrews | D-mandelonitrile + beta-D-glucose | - |
? | |
salicin + H2O | 4% of activity with p-nitrophenyl-beta-D-glucopyranoside | Vanilla planifolia | beta-D-glucose + salicylic alcohol | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 50000, SDS-PAGE | Vanilla planifolia |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Vanilla planifolia |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 70 | approx. 25% of maximal activity at 20°C, approx. 15% of maximal activity at 70°C | Vanilla planifolia |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 40 | no loss of activity after 30 min | Vanilla planifolia |
50 | - |
approx. 40% loss of activity after 30 min | Vanilla planifolia |
60 | - |
approx. 80% loss of activity after 30 min | Vanilla planifolia |
70 | - |
complete loss of activity after 30 min | Vanilla planifolia |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
very sharp optimum | Vanilla planifolia |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 9 | approx. 5% of maximal activity at pH 5.5 and pH 9.0, respectively | Vanilla planifolia |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 12 | complete loss of activity below pH 6.0 after 4 h at 20°C, 37% loss of activity after 4 h at pH 12.0 and 20°C | Vanilla planifolia |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.15 | - |
1-deoxynojirimycin | 40°C, pH 7.0 | Vanilla planifolia | |
0.67 | - |
glucono-delta-lactone | 40°C, pH 7.0 | Vanilla planifolia |