Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.21 extracted from

  • Steenbakkers, P.J.M.; Harhangi, H.R.; Bosscher, M.W.; van der Hooft, M.M.C.; Keltjens, J.T.; van der Drift, C.; Vogels, G.D.; Op den Camp, H.J.M.
    beta-Glucosidase in cellulosome of the anaerobic fungus Piromyces sp. strain E2 is a family 3 glycoside hydrolase (2003), Biochem. J., 370, 963-970.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.21

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Piromyces sp.

Inhibitors

Inhibitors Comment Organism Structure
beta-D-glucose 30 mM, 50% inhibition Piromyces sp.
D-glucono-1,5-lactone 0.1 mM, 50% inhibition Piromyces sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.6
-
 p-nitrophenyl-beta-D-glucopyranoside 40°C, pH 6.0 Piromyces sp.
1.8
-
 cellobiose 40°C, pH 6.0 Piromyces sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
cellulosome
-
 Piromyces sp. 43263
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
91457
-
 x * 91457, deduced from nucleotide sequence Piromyces sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
beta-D-glucosyl-cellulose + H2O Piromyces sp.
-
 cellulose + beta-D-glucose
-
 ?

Organism

Organism UniProt Comment Textmining
Piromyces sp. Q875K3
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant Cel3A Piromyces sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-D-glucosyl-cellulose + H2O
-
 Piromyces sp. cellulose + beta-D-glucose
-
 ?
cellobiose + H2O
-
 Piromyces sp. 2 beta-D-glucose
-
 ?
p-nitrophenyl-beta-D-cellobiose + H2O
-
 Piromyces sp. p-nitrophenyl-beta-D-glucopyranoside + beta-D-glucose
-
 ?
p-nitrophenyl-beta-D-glucopyranoside + H2O
-
 Piromyces sp. p-nitrophenol + beta-D-glucose
-
 ?
p-nitrophenyl-beta-D-xylopyranoside + H2O 1% of activity with p-nitrophenyl-beta-D-glucopyranoside Piromyces sp. p-nitrophenol + beta-D-xylose
-
 ?

Subunits

Subunits Comment Organism
? x * 91457, deduced from nucleotide sequence Piromyces sp.

Synonyms

Synonyms Comment Organism
Cel3A
-
 Piromyces sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Piromyces sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Piromyces sp.

pI Value

Organism Comment pI Value Maximum pI Value
Piromyces sp. deduced from nucleotide sequence
-
 4.9