Inhibitors | Comment | Organism | Structure |
---|---|---|---|
beta-D-glucose | Ki for cellobiase activity: 91 mM, Ki for cell-bound aryl-beta-glucosidase activity: 0.53 mM, Ki for extracellular aryl-beta-glucosidase activity: 0.34 mM | Thermochaetoides thermophila | |
cellobiose | Ki for cellobiase activity: 0.31 mM, Ki for cell-bound aryl-beta-glucosidase activity: 0.72 mM, Ki for extracellular aryl-beta-glucosidase activity: 0.56 mM | Thermochaetoides thermophila | |
p-chloromercuribenzoic acid | sensitivity only for cellobiase activity | Thermochaetoides thermophila |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.076 | - |
4-nitrophenyl-beta-D-glucopyranoside | for extracellular aryl-beta-D-glucosidase | Thermochaetoides thermophila | |
0.089 | - |
4-nitrophenyl-beta-D-glucopyranoside | for cell-bound aryl-beta-D-glucosidase | Thermochaetoides thermophila | |
1.1 | - |
4-nitrophenyl-beta-D-glucopyranoside | cellobiase | Thermochaetoides thermophila |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cell associated | one cellobiase and one aryl-beta-glucosidase | Thermochaetoides thermophila | - |
- |
extracellular | aryl-beta-glucosidase | Thermochaetoides thermophila | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-O-beta-D-glucopyranosyl-D-glucose + H2O | Thermochaetoides thermophila | cellobiose | beta-D-glucose + D-glucose | - |
? | |
4-O-beta-D-glucopyranosyl-D-glucose + H2O | Thermochaetoides thermophila | no activity for aryl-beta-D-glucosidases | beta-D-glucose + D-glucose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermochaetoides thermophila | - |
var. coprophile n. var. | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | 3 different forms, one with cellobiose-activity, two with exclusive aryl-beta-glucosidase activity | Thermochaetoides thermophila | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Thermochaetoides thermophila | 4-nitrophenol + D-glucopyranose | - |
? | |
4-O-beta-D-glucopyranosyl-D-glucose + H2O | cellobiose | Thermochaetoides thermophila | beta-D-glucose + D-glucose | - |
? | |
4-O-beta-D-glucopyranosyl-D-glucose + H2O | no activity for aryl-beta-D-glucosidases | Thermochaetoides thermophila | beta-D-glucose + D-glucose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
aryl-beta-D-glucosidase | - |
Thermochaetoides thermophila |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
54 | - |
completely stable cell-bound cellobiase, cell-bound aryl-beta-glucosidase loses 50% of its activity in 5 h | Thermochaetoides thermophila |