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Literature summary for 3.2.1.21 extracted from

  • Ohmiya, K.; Shirai, M.; Kurachi, Y.; Shimizu, S.
    Isolation and properties of beta-glucosidase from Ruminococcus albus (1985), J. Bacteriol., 161, 432-434.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.21

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol slight activation at 1 mM Ruminococcus albus
Cysteine hydrochloride slight activation at 1 mM Ruminococcus albus
dithiothreitol slight activation at 1 mM Ruminococcus albus
GSH slight activation at 1 mM Ruminococcus albus

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ remarkably inhibition at 1 mM Ruminococcus albus
Hg2+ remarkable inhibition at 1 mM Ruminococcus albus
iodoacetoamide strong inhibition at 1 mM Ruminococcus albus
p-chloromercuribenzoic acid strong inhibition at 1 mM Ruminococcus albus
Zn2+ remarkable inhibition at 1 mM Ruminococcus albus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.2
-
 4-nitrophenyl-beta-D-glucopyranoside
-
 Ruminococcus albus
26
-
 cellobiose
-
 Ruminococcus albus

Localization

Localization Comment Organism GeneOntology No. Textmining
cell associated
-
 Ruminococcus albus
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
82000
-
 gel filtration, SDS-PAGE Ruminococcus albus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4-O-beta-D-glucopyranosyl-D-glucose + H2O Ruminococcus albus cellobiose beta-D-glucose + D-glucose
-
 ?
cellopentaose + 4 H2O Ruminococcus albus
-
 5 D-glucose
-
 ?
cellotetraose + 3 H2O Ruminococcus albus
-
 4 D-glucose
-
 ?
cellotriose + 2 H2O Ruminococcus albus
-
 3 D-glucose
-
 ?

Organism

Organism UniProt Comment Textmining
Ruminococcus albus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein 12% carbohydrate content Ruminococcus albus

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, chromatography steps Ruminococcus albus

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
 Ruminococcus albus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
13
-
-
 Ruminococcus albus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl beta-cellobioside + 2 H2O cleaves beta-glucosidic linkages one by one at the non-reducing end only Ruminococcus albus 4-nitrophenol + 2 beta-D-glucose
-
 ?
4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Ruminococcus albus 4-nitrophenol + D-glucopyranose
-
 ?
4-O-beta-D-glucopyranosyl-D-glucose + H2O cellobiose Ruminococcus albus beta-D-glucose + D-glucose
-
 ?
cellobiose + H2O
-
 Ruminococcus albus 2 beta-D-glucose
-
 ?
cellopentaose + 4 H2O
-
 Ruminococcus albus 5 D-glucose
-
 ?
cellotetraose + 3 H2O
-
 Ruminococcus albus 4 D-glucose
-
 ?
cellotriose + 2 H2O
-
 Ruminococcus albus 3 D-glucose
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 82000, SDS-PAGE Ruminococcus albus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30 35
-
 Ruminococcus albus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
 stable below Ruminococcus albus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
 Ruminococcus albus