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Literature summary for 3.2.1.21 extracted from

  • Chirico, W.J.; Brown, R.D.
    Purification and characterization of a beta-glucosidase from Trichoderma reesei (1987), Eur. J. Biochem., 165, 333-341.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
beta-D-glucose Ki: 0.7 mM Trichoderma reesei

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02
-
4-methylumbelliferyl-beta-D-glucoside
-
Trichoderma reesei
0.102
-
4-nitrophenyl-beta-D-glucopyranoside
-
Trichoderma reesei
1.25
-
cellobiose
-
Trichoderma reesei

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Trichoderma reesei
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
81600
-
SDS-PAGE Trichoderma reesei

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4-O-beta-D-glucopyranosyl-D-glucose + H2O Trichoderma reesei cellobiose beta-D-glucose + D-glucose
-
?

Organism

Organism UniProt Comment Textmining
Trichoderma reesei
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, chromatography steps Trichoderma reesei

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
Trichoderma reesei
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
52
-
-
Trichoderma reesei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl-beta-D-glucoside + H2O
-
Trichoderma reesei 4-methylumbelliferone + beta-D-glucose
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
-
Trichoderma reesei 4-nitrophenol + D-glucopyranose
-
?
4-O-beta-D-glucopyranosyl-D-glucose + H2O cellobiose Trichoderma reesei beta-D-glucose + D-glucose
-
?
cellobiose + H2O
-
Trichoderma reesei 2 beta-D-glucose
-
?

Subunits

Subunits Comment Organism
monomer 1 * 81600, SDS-PAGE Trichoderma reesei

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5 5
-
Trichoderma reesei