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Literature summary for 3.2.1.21 extracted from
- Purification and characterization of a beta-glucosidase from Evernia prunastri (1988), Eur. J. Biochem., 175, 627-632.
General Stability
| General Stability | Organism |
|---|---|
| resistant to thermal inactivation | Evernia prunastri |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| beta-D-glucose | competitive inhibition, Ki: 1.26 mM | Evernia prunastri |  |
| Co2+ | notable inhibition at 10 mM | Evernia prunastri | |
| Fe3+ | notable inhibition at 10 mM | Evernia prunastri | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.24 | - |
cellobiose | - |
Evernia prunastri | |
| 0.64 | - |
4-nitrophenyl-beta-D-glucopyranoside | - |
Evernia prunastri | |
| 1.25 | - |
Salicin | - |
Evernia prunastri | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| intracellular | - |
Evernia prunastri | 5622 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 311000 | - |
gel filtration, native PAGE | Evernia prunastri |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-O-beta-D-glucopyranosyl-D-glucose + H2O | Evernia prunastri | cellobiose | beta-D-glucose + D-glucose | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Evernia prunastri | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| to homogeneity, 2step chromatography | Evernia prunastri |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | - |
Evernia prunastri | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 21 | - |
- |
Evernia prunastri |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (2-hydroxymethylphenyl)-beta-D-glucopyranoside + H2O | salicin | Evernia prunastri | 2-hydroxymethylphenol + beta-D-glucose | - |
? | |
| 4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Evernia prunastri | 4-nitrophenol + D-glucopyranose | - |
? | |
| 4-O-beta-D-glucopyranosyl-D-glucose + H2O | cellobiose | Evernia prunastri | beta-D-glucose + D-glucose | - |
? | |
| cellobiose + H2O | - |
Evernia prunastri | 2 beta-D-glucose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| multimer | composed of several 60-70 kDa-subunits, SDS-PAGE | Evernia prunastri |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Evernia prunastri |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4 | - |
- |
Evernia prunastri |
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