Inhibitors | Comment | Organism | Structure |
---|---|---|---|
beta-D-glucose | Ki: 0.55 mM | Athelia rolfsii | |
D-glucono-1,5-lactone | Ki: 0.01 mM | Athelia rolfsii | |
nojirimycin bisulfite | Ki: 0.001 mM | Athelia rolfsii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.79 | - |
4-nitrophenyl-beta-D-glucopyranoside | beta-glucosidase 4 | Athelia rolfsii | |
0.89 | - |
4-nitrophenyl-beta-D-glucopyranoside | beta-glucosidase 3 | Athelia rolfsii | |
1.07 | - |
4-nitrophenyl-beta-D-glucopyranoside | beta-glucosidase 1 | Athelia rolfsii | |
1.38 | - |
4-nitrophenyl-beta-D-glucopyranoside | beta-glucosidase 2 | Athelia rolfsii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
90000 | - |
beta-glucosidases 1 and 2, gel filtration | Athelia rolfsii |
92000 | - |
beta-glucosidase 4, gel filtration | Athelia rolfsii |
107000 | - |
beta-glucosidase 3, gel filtration | Athelia rolfsii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-O-beta-D-glucopyranosyl-D-glucopyranose + H2O | Athelia rolfsii | sophorose | beta-D-glucose + D-glucose | - |
? | |
3-O-beta-D-glucopyranosyl-D-glucopyranose + H2O | Athelia rolfsii | laminaribiose | beta-D-glucose + D-glucose | - |
? | |
3-O-beta-D-glucopyranosyl-D-glucopyranose + H2O | Athelia rolfsii | not restricted to the beta-D-1,4-linkage | beta-D-glucose + D-glucose | - |
? | |
4-O-beta-D-glucopyranosyl-D-glucose + H2O | Athelia rolfsii | cellobiose | beta-D-glucose + D-glucose | - |
? | |
6-O-beta-D-glucopyranosyl-D-glucose + H2O | Athelia rolfsii | gentiobiose | beta-D-glucose + D-glucose | - |
? | |
cellohexaose + 5 H2O | Athelia rolfsii | - |
6 D-glucose | - |
? | |
cellopentaose + 4 H2O | Athelia rolfsii | preferred substrate | 5 D-glucose | - |
? | |
cellotetraose + 3 H2O | Athelia rolfsii | - |
4 D-glucose | - |
? | |
cellotriose + 2 H2O | Athelia rolfsii | - |
3 D-glucose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Athelia rolfsii | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Athelia rolfsii |
Purification (Comment) | Organism |
---|---|
to homogeneity, 4 different forms, chromatography steps | Athelia rolfsii |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | - |
Athelia rolfsii | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
25 | 57 | 4 different forms | Athelia rolfsii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-nitrophenyl beta-D-glucopyranoside + H2O | - |
Athelia rolfsii | 2-nitrophenol + D-glucopyranose | - |
? | |
2-O-beta-D-glucopyranosyl-D-glucopyranose + H2O | sophorose | Athelia rolfsii | beta-D-glucose + D-glucose | - |
? | |
3-O-beta-D-glucopyranosyl-D-glucopyranose + H2O | laminaribiose | Athelia rolfsii | beta-D-glucose + D-glucose | - |
? | |
3-O-beta-D-glucopyranosyl-D-glucopyranose + H2O | not restricted to the beta-D-1,4-linkage | Athelia rolfsii | beta-D-glucose + D-glucose | - |
? | |
4-nitrophenyl beta-cellobioside + 2 H2O | - |
Athelia rolfsii | 4-nitrophenol + 2 beta-D-glucose | - |
? | |
4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Athelia rolfsii | 4-nitrophenol + D-glucopyranose | - |
? | |
4-O-beta-D-glucopyranosyl-D-glucose + H2O | cellobiose | Athelia rolfsii | beta-D-glucose + D-glucose | - |
? | |
6-O-beta-D-glucopyranosyl-D-glucose + H2O | gentiobiose | Athelia rolfsii | beta-D-glucose + D-glucose | - |
? | |
cellohexaose + 5 H2O | - |
Athelia rolfsii | 6 D-glucose | - |
? | |
cellopentaose + 4 H2O | preferred substrate | Athelia rolfsii | 5 D-glucose | - |
? | |
cellotetraose + 3 H2O | - |
Athelia rolfsii | 4 D-glucose | - |
? | |
cellotriose + 2 H2O | - |
Athelia rolfsii | 3 D-glucose | - |
? | |
methyl-beta-D-glucoside + H2O | - |
Athelia rolfsii | beta-D-glucose + methanol | - |
? | |
phenyl-beta-D-glucoside + H2O | - |
Athelia rolfsii | phenol + beta-D-glucose | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 90000-107000, SDS-PAGE, 4 isoforms | Athelia rolfsii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.2 | - |
for 4 different forms | Athelia rolfsii |