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Literature summary for 3.2.1.21 extracted from

  • Drider, D.; Pommares, P.; Chemardin, P.; Arnaud, A.; Galzy, P.
    Purification and properties of the endocellular beta-glucosidase from Candida cacaoi Buckley and Van Uhden CBS 2020 (1993), J. Appl. Bacteriol., 74, 473-479.
    View publication on PubMed
Search for more literature for 3.2.1.21

Inhibitors

Inhibitors Comment Organism Structure
Ag2+ strong inhibition at 10 mM Millerozyma farinosa
beta-D-glucose competitive inhibition, Ki: 8 mM Millerozyma farinosa
Cu2+ moderate inhibition at 10 mM Millerozyma farinosa
Fe3+ moderate inhibition at 10 mM Millerozyma farinosa
Hg2+ strong inhibition at 10 mM Millerozyma farinosa
Mg2+ moderate inhibition at 10 mM Millerozyma farinosa
Mn2+ moderate inhibition at 10 mM Millerozyma farinosa
N-bromosuccinimide inhibition at 10 mM Millerozyma farinosa
p-chloromercuribenzoic acid inhibition at 1 mM Millerozyma farinosa
Ru2+ moderate inhibition at 10 mM Millerozyma farinosa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.44
-
 4-nitrophenyl-beta-D-glucopyranoside
-
 Millerozyma farinosa

Localization

Localization Comment Organism GeneOntology No. Textmining
intracellular stimulation of enzyme synthesis by cellobiose as carbohydrate source Millerozyma farinosa 5622
-

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ activation at 10 mM Millerozyma farinosa
Na+ activation at 10 mM Millerozyma farinosa
Ni2+ activation at 10 mM Millerozyma farinosa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
110000
-
 2 * 110000, SDS-PAGE Millerozyma farinosa
220000
-
 gel filtration Millerozyma farinosa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-O-beta-D-glucopyranosyl-D-glucopyranose + H2O Millerozyma farinosa low activity beta-D-glucose + D-glucose
-
 ?
2-O-beta-D-glucopyranosyl-D-glucopyranose + H2O Millerozyma farinosa sophorose beta-D-glucose + D-glucose
-
 ?
3-O-beta-D-glucopyranosyl-D-glucopyranose + H2O Millerozyma farinosa laminaribiose beta-D-glucose + D-glucose
-
 ?
4-O-beta-D-glucopyranosyl-D-glucose + H2O Millerozyma farinosa cellobiose beta-D-glucose + D-glucose
-
 ?
4-O-beta-D-glucopyranosyl-D-glucose + H2O Millerozyma farinosa low activity beta-D-glucose + D-glucose
-
 ?
6-O-beta-D-glucopyranosyl-D-glucose + H2O Millerozyma farinosa low activity beta-D-glucose + D-glucose
-
 ?
6-O-beta-D-glucopyranosyl-D-glucose + H2O Millerozyma farinosa gentiobiose beta-D-glucose + D-glucose
-
 ?
alpha-L-arabinofuranose + H2O Millerozyma farinosa low activity alpha-L-arabinofuranose + ?
-
 ?
alpha-L-rhamnopyranoside + H2O Millerozyma farinosa
-
 alpha-L-rhamnopyranose + ?
-
 ?
trehalose + H2O Millerozyma farinosa
-
 beta-D-glucose + ?
-
 ?

Organism

Organism UniProt Comment Textmining
Millerozyma farinosa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, 2step chromatography Millerozyma farinosa

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
 Millerozyma farinosa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
38.63
-
-
 Millerozyma farinosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2-hydroxymethylphenyl)-beta-D-glucopyranoside + H2O salicin Millerozyma farinosa 2-hydroxymethylphenol + beta-D-glucose
-
 ?
(4-hydroxyphenyl)-beta-D-glucopyranoside + H2O arbutin Millerozyma farinosa 4-hydroxyphenol + beta-D-glucopyranose
-
 ?
2-nitrophenyl beta-D-glucopyranoside + H2O
-
 Millerozyma farinosa 2-nitrophenol + D-glucopyranose
-
 ?
2-O-beta-D-glucopyranosyl-D-glucopyranose + H2O low activity Millerozyma farinosa beta-D-glucose + D-glucose
-
 ?
2-O-beta-D-glucopyranosyl-D-glucopyranose + H2O sophorose Millerozyma farinosa beta-D-glucose + D-glucose
-
 ?
3-O-beta-D-glucopyranosyl-D-glucopyranose + H2O laminaribiose Millerozyma farinosa beta-D-glucose + D-glucose
-
 ?
4-nitrophenyl beta-D-fucoside + H2O
-
 Millerozyma farinosa 4-nitrophenol + beta-D-fucose
-
 ?
4-nitrophenyl beta-D-galactoside + H2O
-
 Millerozyma farinosa 4-nitrophenol + beta-D-galactose
-
 ?
4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Millerozyma farinosa 4-nitrophenol + D-glucopyranose
-
 ?
4-nitrophenyl-alpha-D-glucoside
-
 Millerozyma farinosa 4-nitrophenol + alpha-D-glucose
-
 ?
4-O-beta-D-glucopyranosyl-D-glucose + H2O cellobiose Millerozyma farinosa beta-D-glucose + D-glucose
-
 ?
4-O-beta-D-glucopyranosyl-D-glucose + H2O low activity Millerozyma farinosa beta-D-glucose + D-glucose
-
 ?
6-O-beta-D-glucopyranosyl-D-glucose + H2O low activity Millerozyma farinosa beta-D-glucose + D-glucose
-
 ?
6-O-beta-D-glucopyranosyl-D-glucose + H2O gentiobiose Millerozyma farinosa beta-D-glucose + D-glucose
-
 ?
alpha-L-arabinofuranose + H2O low activity Millerozyma farinosa alpha-L-arabinofuranose + ?
-
 ?
alpha-L-rhamnopyranoside + H2O
-
 Millerozyma farinosa alpha-L-rhamnopyranose + ?
-
 ?
methyl-beta-D-glucoside + H2O low activity Millerozyma farinosa beta-D-glucose + methanol
-
 ?
trehalose + H2O
-
 Millerozyma farinosa beta-D-glucose + ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 110000, SDS-PAGE Millerozyma farinosa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Millerozyma farinosa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
 Millerozyma farinosa