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Literature summary for 3.2.1.21 extracted from
- Properties of an intracellular beta-glucosidase purified from the cellobiose-fermenting yeast Candida wickerhamii (1996), Appl. Microbiol. Biotechnol., 546, 353-359.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 1-butanol | 1.6fold increase of activity | Nakazawaea wickerhamii |  |
| 1-Pentanol | 1.6fold increase of activity | Nakazawaea wickerhamii | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-butanol | highest decrease of activity in comparison to ethanol and 2-propanol | Nakazawaea wickerhamii | |
| 2-propanol | higher decrease of activity than for ethanol | Nakazawaea wickerhamii | |
| beta-D-glucose | end-product inhibition, 42% inhibition at 100 mM | Nakazawaea wickerhamii | |
| ethanol | decrease of activity | Nakazawaea wickerhamii | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.28 | - |
4-nitrophenyl-beta-D-glucopyranoside | - |
Nakazawaea wickerhamii | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| intracellular | stimulation of enzyme synthesis by cellobiose as carbohydrate source | Nakazawaea wickerhamii | 5622 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 94000 | - |
2 * 94000, SDS-PAGE | Nakazawaea wickerhamii |
| 180000 | - |
gel filtration | Nakazawaea wickerhamii |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-O-beta-D-galactopyranosyl-D-glucose + H2O | Nakazawaea wickerhamii | lactose | beta-D-galactose + D-glucose | - |
? | |
| 4-O-beta-D-glucopyranosyl-D-glucose + H2O | Nakazawaea wickerhamii | cellobiose | beta-D-glucose + D-glucose | - |
? | |
| trehalose + H2O | Nakazawaea wickerhamii | - |
beta-D-glucose + ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Nakazawaea wickerhamii | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| to homogeneity, chromatography steps | Nakazawaea wickerhamii |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | - |
Nakazawaea wickerhamii | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 10.1 | - |
- |
Nakazawaea wickerhamii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl beta-D-glucopyranoside + H2O | highest activity against aryl-beta-1,4-glucosides | Nakazawaea wickerhamii | 4-nitrophenol + D-glucopyranose | - |
? | |
| 4-nitrophenyl beta-D-xylopyranoside + H2O | - |
Nakazawaea wickerhamii | 4-nitrophenol + beta-D-xylose | - |
? | |
| 4-O-beta-D-galactopyranosyl-D-glucose + H2O | lactose | Nakazawaea wickerhamii | beta-D-galactose + D-glucose | - |
? | |
| 4-O-beta-D-glucopyranosyl-D-glucose + H2O | cellobiose | Nakazawaea wickerhamii | beta-D-glucose + D-glucose | - |
? | |
| trehalose + H2O | - |
Nakazawaea wickerhamii | beta-D-glucose + ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 94000, SDS-PAGE | Nakazawaea wickerhamii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
- |
Nakazawaea wickerhamii |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 28 | - |
labile above | Nakazawaea wickerhamii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 6.5 | - |
Nakazawaea wickerhamii |
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