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Literature summary for 3.2.1.21 extracted from

  • Abe, M.; Higashi, S.
    beta-Glucosidase and beta-galactosidase from the periplasmic space of Rhizobium trifolii cells (1982), J. Gen. Appl. Microbiol., 28, 551-562.
No PubMed abstract available
Search for more literature for 3.2.1.21

Inhibitors

Inhibitors Comment Organism Structure
Co2+ 50% inhibition at 8.5 mM Rhizobium leguminosarum
Cu2+ 98% inhibition at 1 mM Rhizobium leguminosarum
Fe2+ complete inhibition at 1 mM Rhizobium leguminosarum
Hg2+ 95% inhibition at 1 mM Rhizobium leguminosarum
p-chloromercuribenzoic acid complete inhibition at 0.085 mM Rhizobium leguminosarum
Zn2+ 84% inhibition at 1 mM Rhizobium leguminosarum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.003
-
 4-nitrophenyl-beta-D-glucopyranoside
-
 Rhizobium leguminosarum

Localization

Localization Comment Organism GeneOntology No. Textmining
periplasmic space
-
 Rhizobium leguminosarum 42597
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
74000
-
 gel filtration Rhizobium leguminosarum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-O-beta-D-glucopyranosyl-D-glucopyranose + H2O Rhizobium leguminosarum sophorose beta-D-glucose + D-glucose
-
 ?
3-O-beta-D-glucopyranosyl-D-glucopyranose + H2O Rhizobium leguminosarum laminaribiose beta-D-glucose + D-glucose
-
 ?
6-O-beta-D-glucopyranosyl-D-glucose + H2O Rhizobium leguminosarum gentiobiose beta-D-glucose + D-glucose
-
 ?
laminarin + H2O Rhizobium leguminosarum low activity beta-D-glucose + ?
-
 ?

Organism

Organism UniProt Comment Textmining
Rhizobium leguminosarum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
near homogeneity, chromatography techniques Rhizobium leguminosarum

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
 Rhizobium leguminosarum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2-hydroxymethylphenyl)-beta-D-glucopyranoside + H2O salicin Rhizobium leguminosarum 2-hydroxymethylphenol + beta-D-glucose
-
 ?
(4-hydroxyphenyl)-beta-D-glucopyranoside + H2O low activity Rhizobium leguminosarum 4-hydroxyphenol + beta-D-glucopyranose
-
 ?
(4-hydroxyphenyl)-beta-D-glucopyranoside + H2O arbutin Rhizobium leguminosarum 4-hydroxyphenol + beta-D-glucopyranose
-
 ?
(R)-alpha-[(6-O-beta-D-glucopyranosyl-beta-D-glucopyranosyl)oxy]benzeneacetonitrile + H2O amygdalin Rhizobium leguminosarum HCN + ?
-
 ?
2-O-beta-D-glucopyranosyl-D-glucopyranose + H2O sophorose Rhizobium leguminosarum beta-D-glucose + D-glucose
-
 ?
3-O-beta-D-glucopyranosyl-D-glucopyranose + H2O laminaribiose Rhizobium leguminosarum beta-D-glucose + D-glucose
-
 ?
4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Rhizobium leguminosarum 4-nitrophenol + D-glucopyranose
-
 ?
4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Rhizobium leguminosarum 4S 4-nitrophenol + D-glucopyranose
-
 ?
6-O-beta-D-glucopyranosyl-D-glucose + H2O gentiobiose Rhizobium leguminosarum beta-D-glucose + D-glucose
-
 ?
beta-1,2-glucan + H2O low activity Rhizobium leguminosarum beta-D-glucose + ?
-
 ?
CM-cellulose + H2O low activity Rhizobium leguminosarum beta-D-glucose + ?
-
 ?
esculin + H2O
-
 Rhizobium leguminosarum beta-D-glucose + 6,7-dihydroxycoumarin
-
 ?
laminarin + H2O low activity Rhizobium leguminosarum beta-D-glucose + ?
-
 ?
luteose + H2O low activity Rhizobium leguminosarum beta-D-glucose + ?
-
 ?
methyl-beta-D-glucoside + H2O low activity Rhizobium leguminosarum beta-D-glucose + methanol
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
53
-
-
 Rhizobium leguminosarum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 stable up to Rhizobium leguminosarum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.4
-
-
 Rhizobium leguminosarum

pH Range

pH Minimum pH Maximum Comment Organism
5 7.6 retains more than 80% of its activity Rhizobium leguminosarum