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Literature summary for 3.2.1.209 extracted from

  • Mora-Montes, H.M.; Lopez-Romero, E.; Zinker, S.; Ponce-Noyola, P.; Flores-Carreon, A.
    Heterologous expression and biochemical characterization of an alpha1,2-mannosidase encoded by the Candida albicans MNS1 gene (2008), Mem. Inst. Oswaldo Cruz, 103, 724-730.
    View publication on PubMed
Search for more literature for 3.2.1.209

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Candida albicans

Inhibitors

Inhibitors Comment Organism Structure
1-deoxymannojirimycin
-
 Candida albicans
swainsonine
-
 Candida albicans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.07
-
 4-methylumbelliferyl-alpha-D-mannopyranoside
-
 Candida albicans

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum
-
 Candida albicans 5783
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
65000
-
 x * 65000, SDS-PAGE Candida albicans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Candida albicans enzyme is involved in the processing of N-linked mannans ?
-
 ?

Organism

Organism UniProt Comment Textmining
Candida albicans Q8J0Q0 fragment; isoform Mns1
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl-alpha-D-mannopyranoside + H2O
-
 Candida albicans 4-methylumbelliferone + D-mannopyranose
-
 ?
Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)[Manalpha(1-2)Manalpha(1-6)[Manalpha(1-2)Manalpha(1-3)]Manalpha(1-6)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
-
 Candida albicans Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)[Manalpha(1-2)Manalpha(1-6)[Manalpha(1-3)]Manalpha(1-6)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + D-mannose
-
 ?
additional information enzyme is involved in the processing of N-linked mannans Candida albicans ?
-
 ?
additional information no substrate: Man5glcNAc2-Asn Candida albicans ?
-
 ?

Subunits

Subunits Comment Organism
? x * 65000, SDS-PAGE Candida albicans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Candida albicans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Candida albicans

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.019
-
-
 Candida albicans 1-deoxymannojirimycin
0.62
-
-
 Candida albicans swainsonine