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Literature summary for 3.2.1.209 extracted from
- Purification of soluble alpha1,2-mannosidase from Candida albicans CAI-4 (2006), FEMS Microbiol. Lett., 256, 50-56.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1-deoxymannojirimycin | DMJ, inhibition of 4-methylumbelliferyl-alpha-D-mannopyranoside hydrolysis | Candida albicans |  |
| pepstatin A | in the presence of mixed membrane fraction from strain ATTC 26555, the 52 kDa polypeptide is converted into a 43 kDa active product. Protease inhibitors, such as phenylmethylsulphonylfluoride, trans-epoxysuccinyl-L-leucylamido(4-guanidino)butane and 1,10-phenantroline, fail to prevent proteolysis. Pepstatin A, an aspartyl protease inhibitor, fully blocked enzyme processing | Candida albicans | |
| swainsonine | SW, inhibition of 4-methylumbelliferyl-alpha-D-mannopyranoside hydrolysis | Candida albicans | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.09 | - |
4-methylumbelliferyl-alpha-D-mannopyranoside | hydrolysis of the fluorogenic substrate follows hyperbolic kinetics | Candida albicans | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 27000 | - |
analytical electrophoresis of the purified preparation reveals two polypeptides | Candida albicans |
| 43000 | - |
converting the 52 kDa alpha-mannosidase into a polypeptide of 43 kDa retaining full enzyme activity demonstrated in membranes of ATCC 26555, support a precursor-product relationship between soluble alpha1,2-mannosidase E-I (52 kDa) and alpha1,2-mannosidase E-II (43 kDa) | Candida albicans |
| 52000 | - |
analytical electrophoresis of the purified preparation reveals two polypeptides. Responsible for enzyme activity. Active on the fluorogenic substrate 4-methylumbelliferyl-alpha-D-mannopyranoside as revealed by zymogram analysis | Candida albicans |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Candida albicans | - |
strains CAI-4 and ATCC 26555 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ion exchange chromatography, used to measure protein and enzyme activity, Sepharose CL6B, DEAE Bio-Gel A, Sephadex G-25, DEAE Bio-Gel A, Con A-Sepharose, Sephadex G-25 | Candida albicans |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
hydrolysis of the fluorogenic substrate 4-methylumbelliferyl-alpha-D-mannopyranoside follows hyperbolic kinetics and LineweaverBurk plots reveals Vmax values of 30.9 nmol of methylumbelliferone min-1 mg-1 of protein | Candida albicans |
| 0.00003 | - |
purification of soluble alpha-mannosidase from CAI-4 strain, High-speed supernatant used to measure alpha-mannosidase activity | Candida albicans |
| 0.00008 | - |
purification of soluble alpha-mannosidase from CAI-4 strain, Sepharose CL6B | Candida albicans |
| 0.0002 | - |
mixed membrane fraction (MMF) of ATCC 26555 supernatant, expressed as nanomol of methylumbelliferone min-1mg-1 of protein | Candida albicans |
| 0.0005 | - |
mixed membrane fraction (MMF) of CAI-4 and ATCC 26555 plus 1 mM pepstatin supernatant, expressed as nanomol of methylumbelliferone min-1mg-1 of protein | Candida albicans |
| 0.0005 | - |
mixed membrane fraction (MMF) of CAI-4 supernatant, expressed as nanomol of methylumbelliferone min-1mg-1 of protein | Candida albicans |
| 0.0012 | - |
mixed membrane fraction (MMF) of CAI-4 and ATCC 26555 pellet, expressed as nanomol of methylumbelliferone min-1mg-1 of protein | Candida albicans |
| 0.0019 | - |
mixed membrane fraction (MMF) of ATCC 26555 pellet, expressed as nanomol of methylumbelliferone min-1mg-1 of protein | Candida albicans |
| 0.0021 | - |
purification of soluble alpha-mannosidase from CAI-4 strain, DEAE Bio-Gel A | Candida albicans |
| 0.0022 | - |
mixed membrane fraction (MMF) of CAI-4 and ATCC 26555 plus 1 mM pepstatin pellet, expressed as nanomol of methylumbelliferone min-1mg-1 of protein | Candida albicans |
| 0.0026 | - |
mixed membrane fraction (MMF) of CAI-4 pellet, expressed as nanomol of methylumbelliferone min-1mg-1 of protein | Candida albicans |
| 0.003 | - |
purification of soluble alpha-mannosidase from CAI-4 strain, Sephadex G-25 | Candida albicans |
| 0.0047 | - |
mixed membrane fraction (MMF) of CAI-4 and ATCC 26555 supernatant, expressed as nanomol of methylumbelliferone min-1mg-1 of protein | Candida albicans |
| 0.0057 | - |
purification of soluble alpha-mannosidase from CAI-4 strain, DEAE Bio-Gel A | Candida albicans |
| 0.0101 | - |
purification of soluble alpha-mannosidase from CAI-4 strain, Con A-Sepharose | Candida albicans |
| 0.0108 | - |
purification of soluble alpha-mannosidase from CAI-4 strain, Sephadex G-25 | Candida albicans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-methylumbelliferyl-alpha-D-mannopyranoside + H2O | MUaMan fluorogenic substrate to measure enzyme activity | Candida albicans | 4-methylumbelliferone + D-mannose | - |
? | |
| alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,6)]-alpha-D-mannopyranosyl-(1,6)]-beta-D-mannopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranose + H2O | to measure enzyme activity | Candida albicans | ? + D-mannose | - |
? | |
| alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,6)]-alpha-D-mannopyranosyl-(1,6)]-beta-D-mannopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranose + H2O | to measure enzyme activity | Candida albicans | ? + D-mannose | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| alpha1,2-mannosidase | - |
Candida albicans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
optimum activity | Candida albicans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
optimum activity | Candida albicans |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.23 | - |
inhibition of 4-methylumbelliferyl-alpha-D-mannopyranoside hydrolysis | Candida albicans | 1-deoxymannojirimycin | |
| 0.47 | - |
inhibition of 4-methylumbelliferyl-alpha-D-mannopyranoside hydrolysis | Candida albicans | swainsonine | |
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