Literature summary for 3.2.1.209 extracted from

Mora-Montes, H.M.; Lopez-Romero, E.; Zinker, S.; Ponce-Noyola, P.; Flores-Carreon, A.
Conversion of alpha1,2-mannosidase E-I from Candida albicans to alpha1,2-mannosidase E-II by limited proteolysis (2008), Antonie van Leeuwenhoek, 93, 61-69.

Search for more literature for 3.2.1.209

Inhibitors

Inhibitors	Comment	Organism	Structure
1-deoxymannojirimycin	trimming is inhibited preferentially by 1-deoxymannojirimycin, inhibits hydrolysis of 4-methylumbelliferyl-alpha-D-mannopyranoside	Candida albicans
swainsonine	inhibits hydrolysis of 4-methylumbelliferyl-alpha-D-mannopyranoside	Candida albicans

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.11	-	4-methylumbelliferyl-alpha-D-mannopyranoside	MUaMan, fluorogenic substrate. Vmax 36.2 nmol of 4-methylumbelliferone min-1 mg-1 of protein	Candida albicans

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
23000	-	analytical electrophoresis of the purified enzyme reveals two polypeptides of 52 and 23 kDa, 52000 Da polypeptide being responsible for enzyme activity as revealed by zymogram analysis	Candida albicans
43000	-	Candida albicans CAI-4 mutant generating an active 43 kDa polypeptide. Zymogram analysis shows that the single proteolytic product of 43 kDa is able to hydrolyze the fluorogenic substrate and, this is not observed when proteolysis is blocked by pepstatin	Candida albicans
43000	-	purified alpha-mannosidase E-II remains unaltered after treatment with aspartyl protease and retains full activity on artificial substrate 4-methylumbelliferyl-alpha-D-mannopyranoside	Candida albicans
52000	-	alpha1,2-mannosidase E-I. Analytical electrophoresis of the purified enzyme reveals two polypeptides of 52 and 23 kDa, 52000 Da polypeptide being responsible for enzyme activity as revealed by zymogram analysis	Candida albicans

Organism

Organism	UniProt	Comment	Textmining
Candida albicans	-	ATCC 26555	-

Purification (Commentary)

Purification (Comment)	Organism
by conventional methods of protein isolation and affinity chromatography in Concanavalin A-Sepharose 4B. Alpha-mannosidase E-I is purified by a fungal aspartyl protease	Candida albicans

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-methylumbelliferyl-alpha-D-mannopyranoside	MUaMan, fluorogenic substrate	Candida albicans	4-methylumbelliferone + D-mannose	-	?

Synonyms

Synonyms	Comment	Organism
alpha1,2-mannosidase	-	Candida albicans
alpha1,2-mannosidase E-I	-	Candida albicans
alpha1,2-mannosidase E-II	-	Candida albicans

IC50 Value

IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor	Structure
0.2	-	inhibits hydrolysis of 4-methylumbelliferyl-alpha-D-mannopyranoside	Candida albicans	1-deoxymannojirimycin
0.55	-	inhibits hydrolysis of 4-methylumbelliferyl-alpha-D-mannopyranoside	Candida albicans	swainsonine

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Release 2023.1 (January 2023)