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Literature summary for 3.2.1.209 extracted from

  • Karaveg, K.; Siriwardena, A.; Tempel, W.; Liu, Z.; Glushka, J.; Wang, B.; Moremen, K.W.
    Mechanism of class 1 (glycosylhydrolase family 47) alpha-mannosidases involved in N-glycan processing and endoplasmic reticulum quality control (2005), J. Biol. Chem., 280, 16197-16207.
    View publication on PubMed
Search for more literature for 3.2.1.209

Crystallization (Commentary)

Crystallization (Comment) Organism
co-complex with an uncleaved thiodisaccharide substrate analog, hanging drop vapor diffusion method Homo sapiens

Protein Variants

Protein Variants Comment Organism
D463N pH-optimum is 6.8 compared to 7.1 for wild-type enzyme, kcat/Km is 212500fold lower than wild-type value Homo sapiens
D463N/E599Q pH-optimum is 6.6 compared to 7.1 for wild-type enzyme, kcat/Km is 8.3fold lower than wild-type value Homo sapiens
E330Q pH-optimum is 5.3 compared to 7.1 for wild-type enzyme, kcat/Km is 809.5fold lower than wild-type value Homo sapiens
E330Q/E599Q pH-optimum is 6.6 compared to 7.1 for wild-type enzyme, kcat/Km is 340000fold lower than wild-type value Homo sapiens
E599Q pH-optimum is 6.7 compared to 7.1 for wild-type enzyme, kcat/Km is 17895fold lower than wild-type value Homo sapiens
H524A pH-optimum is 6.9 compared to 7.1 for wild-type enzyme, kcat/Km is 28.3fold lower than wild-type value Homo sapiens
R334A mutant enzyme is not detected in the culture medium, kcat/Km is 28.3fold lower than wild-type value Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.068
-
 alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->6)]-alpha-D-Man-(1->6)]-alpha-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-2-pyridylamine 37°C, pH 5.3, pyridylamine-tagged Man9GlcNAc2, mutant enzyme E330Q Homo sapiens
0.09
-
 alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->6)]-alpha-D-Man-(1->6)]-alpha-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-2-pyridylamine 37°C, pH 7.1, pyridylamine-tagged Man9GlcNAc2, mutant enzyme E330Q Homo sapiens
0.11
-
 alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->6)]-alpha-D-Man-(1->6)]-alpha-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-2-pyridylamine 37°C, pH 6.8, pyridylamine-tagged Man9GlcNAc2, mutant enzyme D463N Homo sapiens
0.11
-
 alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->6)]-alpha-D-Man-(1->6)]-alpha-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-2-pyridylamine 37°C, pH 7.1, pyridylamine-tagged Man9GlcNAc2, wild-type enzyme Homo sapiens
0.15
-
 alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->6)]-alpha-D-Man-(1->6)]-alpha-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-2-pyridylamine 37°C, pH 6.6, pyridylamine-tagged Man9GlcNAc2, mutant enzyme E330Q/E599Q Homo sapiens
0.16
-
 alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->6)]-alpha-D-Man-(1->6)]-alpha-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-2-pyridylamine 37°C, pH 6.7, pyridylamine-tagged Man9GlcNAc2, mutant enzyme E599Q Homo sapiens
0.21
-
 alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->6)]-alpha-D-Man-(1->6)]-alpha-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-2-pyridylamine 37°C, pH 6.9, pyridylamine-tagged Man9GlcNAc2, mutant enzyme H524A Homo sapiens
0.26
-
 alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->6)]-alpha-D-Man-(1->6)]-alpha-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-2-pyridylamine 37°C, pH 6.6, pyridylamine-tagged Man9GlcNAc2, mutant enzyme D463N/E599Q Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum
-
 Homo sapiens 5783
-

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9UKM7 recombinant
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->6)]-alpha-D-Man-(1->6)]-alpha-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-2-pyridylamine + H2O pyridylamine-tagged Man9GlcNAc2 Homo sapiens alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->6)]-alpha-D-Man-(1->6)]-alpha-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-2-pyridylamine + D-mannopyranose
-
 ?

Synonyms

Synonyms Comment Organism
ERManI
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.000026
-
 alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->6)]-alpha-D-Man-(1->6)]-alpha-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-2-pyridylamine 37°C, pH 6.7, pyridylamine-tagged Man9GlcNAc2, mutant enzyme E599Q Homo sapiens
0.000027
-
 alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->6)]-alpha-D-Man-(1->6)]-alpha-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-2-pyridylamine 37°C, pH 6.6, pyridylamine-tagged Man9GlcNAc2, mutant enzyme D463N/E599Q Homo sapiens
0.00028
-
 alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->6)]-alpha-D-Man-(1->6)]-alpha-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-2-pyridylamine 37°C, pH 6.6, pyridylamine-tagged Man9GlcNAc2, mutant enzyme E330Q/E599Q Homo sapiens
0.0046
-
 alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->6)]-alpha-D-Man-(1->6)]-alpha-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-2-pyridylamine 37°C, pH 6.8, pyridylamine-tagged Man9GlcNAc2, mutant enzyme D463N Homo sapiens
0.018
-
 alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->6)]-alpha-D-Man-(1->6)]-alpha-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-2-pyridylamine 37°C, pH 7.1, pyridylamine-tagged Man9GlcNAc2, mutant enzyme E330Q Homo sapiens
0.084
-
 alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->6)]-alpha-D-Man-(1->6)]-alpha-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-2-pyridylamine 37°C, pH 5.3, pyridylamine-tagged Man9GlcNAc2, mutant enzyme E330Q Homo sapiens
0.86
-
 alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->6)]-alpha-D-Man-(1->6)]-alpha-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-2-pyridylamine 37°C, pH 6.9, pyridylamine-tagged Man9GlcNAc2, mutant enzyme H524A Homo sapiens
3.7
-
 alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->6)]-alpha-D-Man-(1->6)]-alpha-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-2-pyridylamine 37°C, pH 7.1, pyridylamine-tagged Man9GlcNAc2, wild-type enzyme Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.3
-
 mutant enzyme E330Q Homo sapiens
6.6
-
 mutant enzyme E330Q/E599Q and D463N/E599Q Homo sapiens
6.7
-
 mutant enzyme E599Q Homo sapiens
6.8
-
 mutant enzyme D463N Homo sapiens
6.9
-
 mutant enzyme H524A Homo sapiens
7.1
-
 wild-type enzyme Homo sapiens