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Literature summary for 3.2.1.209 extracted from

  • Lipari, F.; Herscovics, A.
    Role of the cysteine residues in the alpha-1,2-mannosidase involved in N-glycan biosynthesis in Saccharomyces cerevisiae. The conserved Cys340 and Cys385 residues form an essential disulfide bond (1996), J. Biol. Chem., 271, 27615-27622.
    View publication on PubMed
Search for more literature for 3.2.1.209

Protein Variants

Protein Variants Comment Organism
C340S no mutant protein detectable Saccharomyces cerevisiae
C385S no mutant protein detectable Saccharomyces cerevisiae
C468S Km is 0.7 mM for (Man)9GlcNAc, same specificity as wild type Saccharomyces cerevisiae
C471S Km is 0.7 mM for (Man)9GlcNAc, same specificity as wild type Saccharomyces cerevisiae
C485S Km is 0.4 mM for (Man)9GlcNAc, same specificity as wild type Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
dithiothreitol loss of activity with first-order kinetics Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.3
-
 (Man)9GlcNAc pH 6.5 Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
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-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
additional information contains two disulfide bonds and one free thiol group, soluble catalytic domain, recombinant enzyme Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->6)]-alpha-D-Man-(1->6)]-alpha-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn-[protein] + H2O
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 Saccharomyces cerevisiae alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->6)]-alpha-D-Man-(1->6)]-alpha-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn-[protein] + D-mannopyranose
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