Literature summary for 3.2.1.209 extracted from

Lipari, F.; Herscovics, A.
Calcium Binding to the Class I alpha-1,2-mannosidase from Saccharomyces cerevisiae occurs outside the EF hand motif (1999), Biochemistry, 38, 1111-1118.

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Protein Variants

Protein Variants	Comment	Organism
D121A	putative EF hand Ca2+ binding loop, no effect on Ca2+ binding	Saccharomyces cerevisiae
D121A/E132V	putative EF hand Ca2+ binding loop, no effect on Ca2+ binding	Saccharomyces cerevisiae
D121N	putative EF hand Ca2+ binding loop, no effect on Ca2+ binding	Saccharomyces cerevisiae
D275N	no ability of Ca2+ binding	Saccharomyces cerevisiae
D86N	retains ability of Ca2+ binding, Km value 0.007 mM	Saccharomyces cerevisiae
E132Q	putative EF hand Ca2+ binding loop, no effect on Ca2+ binding	Saccharomyces cerevisiae
E132Q	retains ability of Ca2+ binding, Km value 0.3 mM	Saccharomyces cerevisiae
E132V	putative EF hand Ca2+ binding loop, no effect on Ca2+ binding	Saccharomyces cerevisiae
E279Q	no ability of Ca2+ binding	Saccharomyces cerevisiae
E438Q	no ability of Ca2+ binding	Saccharomyces cerevisiae
E503Q	retains ability of Ca2+ binding, but completely inactive	Saccharomyces cerevisiae
E526Q	retains ability of Ca2+ binding, Km value 4 mM	Saccharomyces cerevisiae

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	requirement, protects from thermal inactivation, Km value 0.5 mM	Saccharomyces cerevisiae

Organism

Organism	UniProt	Comment	Textmining
Saccharomyces cerevisiae	-	-	-

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Release 2023.1 (January 2023)