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Literature summary for 3.2.1.208 extracted from

  • Alarico, S.; Empadinhas, N.; da Costa, M.
    A new bacterial hydrolase specific for the compatible solutes alpha-D-mannopyranosyl-(1->2)-D-glycerate and alpha-D-glucopyranosyl-(1->2)-D-glycerate (2013), Enzyme Microb. Technol., 52, 77-83 .
    View publication on PubMed
Search for more literature for 3.2.1.208

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.62
-
 2-O-(alpha-D-mannopyranosyl)-D-glycerate pH 4.5, 70°C Thermus thermophilus
3.74
-
 2-O-(alpha-D-glucopyranosyl)-D-glycerate pH 4.5, 70°C Thermus thermophilus
4.18
-
 2-O-(alpha-D-glucopyranosyl)-D-glycerate pH 4.5, 50°C Rubrobacter radiotolerans
6.37
-
 2-O-(alpha-D-mannopyranosyl)-D-glycerate pH 4.5, 50°C Rubrobacter radiotolerans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
92900
-
 gel filtration Rubrobacter radiotolerans
205800
-
 gel filtration Thermus thermophilus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-O-(alpha-D-glucopyranosyl)-D-glycerate + H2O Thermus thermophilus
-
 D-glucopyranose + D-glycerate
-
 ?
2-O-(alpha-D-glucopyranosyl)-D-glycerate + H2O Rubrobacter radiotolerans
-
 D-glucopyranose + D-glycerate
-
 ?
2-O-(alpha-D-mannopyranosyl)-D-glycerate + H2O Thermus thermophilus
-
 D-mannopyranose + D-glycerate
-
 ?
2-O-(alpha-D-mannopyranosyl)-D-glycerate + H2O Rubrobacter radiotolerans
-
 D-mannopyranose + D-glycerate
-
 ?

Organism

Organism UniProt Comment Textmining
Rubrobacter radiotolerans
-
-
-
Thermus thermophilus Q72K04
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-O-(alpha-D-glucopyranosyl)-D-glycerate + H2O
-
 Thermus thermophilus D-glucopyranose + D-glycerate
-
 ?
2-O-(alpha-D-glucopyranosyl)-D-glycerate + H2O
-
 Rubrobacter radiotolerans D-glucopyranose + D-glycerate
-
 ?
2-O-(alpha-D-mannopyranosyl)-D-glycerate + H2O
-
 Thermus thermophilus D-mannopyranose + D-glycerate
-
 ?
2-O-(alpha-D-mannopyranosyl)-D-glycerate + H2O
-
 Rubrobacter radiotolerans D-mannopyranose + D-glycerate
-
 ?
additional information no substrates: mannosylglyceramide, glucosylglycerol, mannosylglucosylglycerate, glucosylglucosylglycerate, 4-nitrophenyl-alpha-D-mannopyranoside and 4-nitrophenyl-alpha-D-glucopyranoside Thermus thermophilus ?
-
 ?
additional information no substrates: mannosylglyceramide, glucosylglycerol, mannosylglucosylglycerate, glucosylglucosylglycerate, 4-nitrophenyl-alpha-D-mannopyranoside and 4-nitrophenyl-alpha-D-glucopyranoside Rubrobacter radiotolerans ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 50400, calculated from sequence Rubrobacter radiotolerans
tetramer 4 * 48100, calculated from sequence Thermus thermophilus

Synonyms

Synonyms Comment Organism
MGH
-
 Thermus thermophilus
MGH
-
 Rubrobacter radiotolerans
RradDRAFT 2972
-
 Rubrobacter radiotolerans
TT_C0614
-
 Thermus thermophilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Rubrobacter radiotolerans
70
-
-
 Thermus thermophilus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30
-
 no activity below Thermus thermophilus
30
-
 no activity below Rubrobacter radiotolerans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
 half-life 16.1 h Thermus thermophilus
80
-
 half-life 2.5 h Thermus thermophilus
90
-
 half-life 0.20 h Thermus thermophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
-
 Thermus thermophilus
4.5
-
-
 Rubrobacter radiotolerans