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Literature summary for 3.2.1.207 extracted from

  • Okuyama, M.; Miyamoto, M.; Matsuo, I.; Iwamoto, S.; Serizawa, R.; Tanuma, M.; Ma, M.; Klahan, P.; Kumagai, Y.; Tagami, T.; Kimura, A.
    Substrate recognition of the catalytic alpha-subunit of glucosidase II from Schizosaccharomyces pombe (2017), Biosci. Biotechnol. Biochem., 81, 1503-1511 .
    View publication on PubMed
Search for more literature for 3.2.1.207

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Schizosaccharomyces pombe

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ 40 mM, 63% loss of activity Schizosaccharomyces pombe
additional information NaCl, NaH2PO4, Na2SO4, NaNO3, KCl, MgCl2, or EDTA up to 40 mM do not affect activity Schizosaccharomyces pombe

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.13
-
 nigerose pH 6.5, 30°C Schizosaccharomyces pombe
15.3
-
 maltohexaose pH 6.5, 30°C Schizosaccharomyces pombe
18
-
 maltose pH 6.5, 30°C Schizosaccharomyces pombe

Organism

Organism UniProt Comment Textmining
Schizosaccharomyces pombe Q9US55 subunit alpha
-
Schizosaccharomyces pombe 972 Q9US55 subunit alpha
-

Storage Stability

Storage Stability Organism
4°C, 2 days, more than 60% loss of activity, presence of 10% v/v glycerol prevents this loss Schizosaccharomyces pombe

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Glc2Man9GlcNAc2 + H2O
-
 Schizosaccharomyces pombe GlcMan9GlcNAc2 + D-glucose
-
 ?
Glc2Man9GlcNAc2 + H2O
-
 Schizosaccharomyces pombe 972 GlcMan9GlcNAc2 + D-glucose
-
 ?
GlcMan9GlcNAc2 + H2O
-
 Schizosaccharomyces pombe Man9GlcNAc2 + D-glucose
-
 ?
maltohexaose + H2O
-
 Schizosaccharomyces pombe maltopentaose + D-glucose
-
 ?
maltohexaose + H2O
-
 Schizosaccharomyces pombe 972 maltopentaose + D-glucose
-
 ?
maltose + H2O
-
 Schizosaccharomyces pombe 2 D-glucose
-
 ?
maltose + H2O
-
 Schizosaccharomyces pombe 972 2 D-glucose
-
 ?
additional information enzyme hydrolyzes alpha-(1->3)- and also alpha-(1->2)-, alpha-(1->4)-, and alpha-(1->6)-glucosidic linkages, and 4-nitrophenyl alpha-D-glucoside Schizosaccharomyces pombe ?
-
 ?
additional information enzyme hydrolyzes alpha-(1->3)- and also alpha-(1->2)-, alpha-(1->4)-, and alpha-(1->6)-glucosidic linkages, and 4-nitrophenyl alpha-D-glucoside Schizosaccharomyces pombe 972 ?
-
 ?
nigerose + H2O
-
 Schizosaccharomyces pombe 2 D-glucose
-
 ?
nigerose + H2O
-
 Schizosaccharomyces pombe 972 2 D-glucose
-
 ?

Synonyms

Synonyms Comment Organism
GLS2
-
 Schizosaccharomyces pombe
SPAC1002.03c
-
 Schizosaccharomyces pombe

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 stable up to Schizosaccharomyces pombe
55
-
 complete inactivation Schizosaccharomyces pombe

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.102
-
 nigerose pH 6.5, 30°C Schizosaccharomyces pombe
0.109
-
 maltohexaose pH 6.5, 30°C Schizosaccharomyces pombe
0.241
-
 maltose pH 6.5, 30°C Schizosaccharomyces pombe

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
 Schizosaccharomyces pombe

pH Stability

pH Stability pH Stability Maximum Comment Organism
6.2 9.1
-
 Schizosaccharomyces pombe

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
7.15
-
 maltohexaose pH 6.5, 30°C Schizosaccharomyces pombe
13.4
-
 maltose pH 6.5, 30°C Schizosaccharomyces pombe
48.1
-
 nigerose pH 6.5, 30°C Schizosaccharomyces pombe