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Literature summary for 3.2.1.207 extracted from
- Molecular cloning and characterization of the alpha-glucosidase II from Bombyx mori and Spodoptera frugiperda (2013), Insect Biochem. Mol. Biol., 43, 319-327.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| recombinant expression of His-tagged alpha-subunits via the vaculovirus transfection system in Sf21AE cells | Spodoptera frugiperda |
| recombinant expression of His-tagged and GFP-tagged alpha-subunit via the vaculovirus transfection system in Sf21AE cells | Bombyx mori |
| recombinant expression of His-tagged and GFP-tagged beta-subunits via the vaculovirus transfection system in Sf21AE cells | Bombyx mori |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,4-dideoxy-1,4-imino-D-arabinitol | - |
Bombyx mori |  |
| 1,4-dideoxy-1,4-imino-D-arabinitol | - |
Spodoptera frugiperda | |
| 1-deoxynojirimycin | - |
Bombyx mori | |
| 1-deoxynojirimycin | - |
Spodoptera frugiperda | |
| castanospermine | - |
Bombyx mori | |
| castanospermine | - |
Spodoptera frugiperda | |
| additional information | the enzyme from silkworm is not sensitive to the alpha-glucosidase inhibitors from mulberry leaves; the enzyme from silkworm is not very sensitive to the alpha-glucosidase inhibitors from mulberry leaves | Bombyx mori |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Michaelis-Menten kinetics | Bombyx mori | |
| additional information | - |
additional information | Michaelis-Menten kinetics | Spodoptera frugiperda | |
| 2.37 | - |
4-nitrophenyl alpha-D-glucopyranoside | recombinant enzyme, pH 6,5, 37°C | Spodoptera frugiperda | |
| 2.82 | - |
4-nitrophenyl alpha-D-glucopyranoside | recombinant enzyme, pH 6,5, 37°C | Bombyx mori | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| endoplasmic reticulum | - |
Bombyx mori | 5783 | - |
| endoplasmic reticulum | - |
Spodoptera frugiperda | 5783 | - |
| additional information | the enzyme has a signal peptide in the N-terminus | Bombyx mori | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 105000 | - |
alphabeta, x * 105000, recombinant alpha-subunit, SDS-PAGE, beta-subunit not specified in the publication | Bombyx mori |
| 105206 | - |
alphabeta, x * 105206, alpha-subunit, sequence calculation, x * 106000, recombinant alpha-subunit, SDS-PAGE, beta-subunit not specified in the publication | Spodoptera frugiperda |
| 106000 | - |
alphabeta, x * 105206, alpha-subunit, sequence calculation, x * 106000, recombinant alpha-subunit, SDS-PAGE, beta-subunit not specified in the publication | Spodoptera frugiperda |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bombyx mori | L8B6C5 | beta-subunit | - |
| Bombyx mori | L8B8U1 | alpha-subunit | - |
| Spodoptera frugiperda | L8B8V1 | alpha-subunit | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged and GFP-tagged subunit by nickel affinity chromatography | Bombyx mori |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| BmN cell | - |
Bombyx mori | - |
| Sf21AE cell | - |
Spodoptera frugiperda | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 4.53 | - |
recombinant enzyme, pH 6,5, 37°C, substrate 4-nitrophenyl alpha-D-glucopyranoside | Bombyx mori |
| 4.65 | - |
recombinant enzyme, pH 6,5, 37°C, substrate 4-nitrophenyl alpha-D-glucopyranoside | Spodoptera frugiperda |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl alpha-D-glucopyranoside + H2O | - |
Spodoptera frugiperda | 4-nitrophenol + alpha-D-glucopyranose | - |
? | |
| 4-nitrophenyl alpha-D-glucopyranoside + H2O | cleavage by the single alpha-subunit, the beta-subunit is not required for activity | Bombyx mori | 4-nitrophenol + alpha-D-glucopyranose | - |
? | |
| additional information | no activity of the single alpha-subunit with N-glycan, the enzyme complex of the alpha- and beta-subunits is required for N-glycan cleavage | Bombyx mori | ? | - |
? | |
| additional information | no activity with N-glycan | Spodoptera frugiperda | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| heterodimer | alphabeta | Bombyx mori |
| heterodimer | alphabeta, x * 105000, recombinant alpha-subunit, SDS-PAGE, beta-subunit not specified in the publication | Bombyx mori |
| heterodimer | alphabeta, x * 105206, alpha-subunit, sequence calculation, x * 106000, recombinant alpha-subunit, SDS-PAGE, beta-subunit not specified in the publication | Spodoptera frugiperda |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| alpha-glucosidase II | - |
Bombyx mori |
| alpha-glucosidase II | - |
Spodoptera frugiperda |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Bombyx mori |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
assay at | Bombyx mori |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.0079 | - |
recombinant enzyme, pH 6,5, 37°C | Spodoptera frugiperda | 1-deoxynojirimycin | |
| 0.0195 | - |
recombinant enzyme, pH 6,5, 37°C | Spodoptera frugiperda | castanospermine | |
| 0.0501 | - |
recombinant enzyme, pH 6,5, 37°C | Bombyx mori | castanospermine | |
| 0.0708 | - |
recombinant enzyme, pH 6,5, 37°C | Spodoptera frugiperda | 1,4-dideoxy-1,4-imino-D-arabinitol | |
| 0.0727 | - |
recombinant enzyme, pH 6,5, 37°C | Bombyx mori | 1-deoxynojirimycin | |
| 0.5102 | - |
recombinant enzyme, pH 6,5, 37°C | Bombyx mori | 1,4-dideoxy-1,4-imino-D-arabinitol | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | the enzyme contains a glycoside hydrolase family 31 motif containing the conserved WXDMNE sequence, which is essential for enzymatic activity | Bombyx mori |
| additional information | high concentration of alpha-glucosidase inhibitors from mulberry leaves accumulate in Bombyx mori by feeding, silkworm does not show any toxic symptom against these inhibitors and N-glycosylation of recombinant proteins is not affected | Bombyx mori |
| physiological function | the enzyme is important for N-glycosylation processing and quality control of nascent glycoproteins | Bombyx mori |
| physiological function | the enzyme is important for N-glycosylation processing and quality control of nascent glycoproteins | Spodoptera frugiperda |
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