Cloned (Comment) | Organism |
---|---|
genes Aogls2alpha and/or Aogls2beta encdoing the alpha- und beta-subunit, cloning and expression in Escherichia coli strain DH5alpha | Aspergillus oryzae |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of strains of gene disruptants lacking either the glucosidase II alpha- or beta-subunit. The mutant lacking the beta-subunit is inactive with both Glc2Man9GlcNAc2 and Glc1Man9GlcNAc2, but activity can be restored by adding the beta-subunit fraction, overview | Aspergillus oryzae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
deoxynojirimycin | an alpha-glucosidase-specific inhibitor | Aspergillus oryzae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
endoplasmic reticulum membrane | - |
Aspergillus oryzae | 5789 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Aspergillus oryzae | glucosidase II is a glycoprotein-processing enzyme that successively cleaves two alpha1,3-linked glucose residues from N-linked oligosaccharides in the endoplasmic reticulum | ? | - |
? | |
additional information | Aspergillus oryzae RIB 40 | glucosidase II is a glycoprotein-processing enzyme that successively cleaves two alpha1,3-linked glucose residues from N-linked oligosaccharides in the endoplasmic reticulum | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus oryzae | - |
- |
- |
Aspergillus oryzae RIB 40 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl alpha-D-glucopyranoside + H2O | - |
Aspergillus oryzae | 4-nitrophenol + D-glucopyranose | - |
? | |
4-nitrophenyl alpha-D-glucopyranoside + H2O | - |
Aspergillus oryzae RIB 40 | 4-nitrophenol + D-glucopyranose | - |
? | |
Glc2Man9GlcNAc2 + H2O | synthetic methotrexate-coupled glycan substrate, G1M9-MTX | Aspergillus oryzae | GlcMan9GlcNAc2 + D-glucopyranose | - |
? | |
Glc2Man9GlcNAc2 + H2O | synthetic methotrexate-coupled glycan substrate, G1M9-MTX | Aspergillus oryzae RIB 40 | GlcMan9GlcNAc2 + D-glucopyranose | - |
? | |
GlcMan9GlcNAc2 + H2O | synthetic methotrexate-coupled glycan substrate, G2M9-MTX | Aspergillus oryzae | Man9GlcNAc2 + D-glucopyranose | - |
? | |
GlcMan9GlcNAc2 + H2O | synthetic methotrexate-coupled glycan substrate, G2M9-MTX | Aspergillus oryzae RIB 40 | Man9GlcNAc2 + D-glucopyranose | - |
? | |
additional information | glucosidase II is a glycoprotein-processing enzyme that successively cleaves two alpha1,3-linked glucose residues from N-linked oligosaccharides in the endoplasmic reticulum | Aspergillus oryzae | ? | - |
? | |
additional information | the mutant lacking the beta-subunit is inactive with both Glc2Man9GlcNAc2 and GlcMan9GlcNAc2 | Aspergillus oryzae | ? | - |
? | |
additional information | glucosidase II is a glycoprotein-processing enzyme that successively cleaves two alpha1,3-linked glucose residues from N-linked oligosaccharides in the endoplasmic reticulum | Aspergillus oryzae RIB 40 | ? | - |
? | |
additional information | the mutant lacking the beta-subunit is inactive with both Glc2Man9GlcNAc2 and GlcMan9GlcNAc2 | Aspergillus oryzae RIB 40 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterodimer | the enzyme is a heterodimer whose alpha-subunit contains a glycosidase active site, while the beta-subunit confers the substrate specificity toward di- and monoglucosylated glycans on the glucose-trimming activity of the alpha-subunit | Aspergillus oryzae |
Synonyms | Comment | Organism |
---|---|---|
glucosidase II | - |
Aspergillus oryzae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Aspergillus oryzae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Aspergillus oryzae |