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Literature summary for 3.2.1.207 extracted from
- Purification and characterization of glucosidase II, an endoplasmic reticulum hydrolase involved in glycoprotein biosynthesis (1982), J. Biol. Chem., 257, 9991-10000.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 2-deoxy-D-glucose | activation substrate p-nitrophenyl-alpha-D-glucoside | Rattus norvegicus |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| D-glucono-1,5-lactone | - |
Rattus norvegicus | |
| glucose | - |
Rattus norvegicus | |
| glycerol | - |
Rattus norvegicus | |
| maltose | - |
Rattus norvegicus | |
| p-chloromercuribenzenesulfonate | - |
Rattus norvegicus | |
| Tris | - |
Rattus norvegicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.85 | - |
p-nitrophenyl alpha-D-glucopyranoside | - |
Rattus norvegicus | |
| 4.8 | - |
maltose | - |
Rattus norvegicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| microsome | - |
Rattus norvegicus | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 260000 | 290000 | gel electrophoresis, gel filtration | Rattus norvegicus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Glc2Man9GlcNAc2 + H2O | Rattus norvegicus | - |
Man9GlcNAc2 + alpha-D-glucose | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rattus norvegicus |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| Glc2Man9GlcNAc2-[protein] + H2O = GlcMan9GlcNAc2-[protein] + beta-D-glucopyranose | Glc2Man9GlcNAc2 + H2O = Man9GlcNAc2 + glucose | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Rattus norvegicus | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -70°C, 100 mM phosphate buffer, pH 7, 100 mM maltose, 5 mM mercaptoethanol, several weeks | Rattus norvegicus |
| 4-6°C, 100 mM phosphate buffer, pH 7, 10% glycerol, 5 mM mercaptoethanol, several days | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl alpha-D-glucopyranoside + H2O | - |
Rattus norvegicus | 4-nitrophenol + D-glucose | - |
? | |
| Glc2Man9GlcNAc2 + 2 H2O | - |
Rattus norvegicus | Man9GlcNAc2 + 2 D-glucose | - |
? | |
| Glc2Man9GlcNAc2 + H2O | - |
Rattus norvegicus | Man9GlcNAc2 + alpha-D-glucose | - |
? | |
| maltose + H2O | - |
Rattus norvegicus | alpha-D-glucose + D-glucose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | SDS-PAGE | Rattus norvegicus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 36 | - |
- |
Rattus norvegicus |
| 40 | - |
inactivation | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.6 | 7 | depending on substrate | Rattus norvegicus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8 | with 5 mM mercaptoethanol | Rattus norvegicus |
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Release 2023.1 (January 2023)
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