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Literature summary for 3.2.1.207 extracted from
- Isolation of a homogeneous glucosidase II from pig kidney microsomes (1984), Eur. J. Biochem., 141, 149-156.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| isomaltose | lowering Km, 142% activation at 50 mM | Sus scrofa |  |
| mannose | increasing Vmax, 172% activation at 10 mM | Sus scrofa | |
| starch | increasing Vmax, 160% activation at 7 mg/ml | Sus scrofa | |
General Stability
| General Stability | Organism |
|---|---|
| glycerol, stabilization | Sus scrofa |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| maltose | - |
Sus scrofa | |
| Tris | - |
Sus scrofa | |
| turanose | - |
Sus scrofa | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.019 | - |
4-methylumbelliferyl alpha-D-glucopyranoside | - |
Sus scrofa | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| endoplasmic reticulum | lumen | Sus scrofa | 5783 | - |
| microsome | - |
Sus scrofa | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 100000 | - |
4 * 100000, SDS-PAGE | Sus scrofa |
| 400000 | - |
non denaturing PAGE | Sus scrofa |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Glc2Man9GlcNAc2 + H2O | Sus scrofa | - |
Man9GlcNAc2 + alpha-D-glucose | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sus scrofa | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | - |
Sus scrofa |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Sus scrofa |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| kidney | - |
Sus scrofa | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.151 | - |
4-methylumbelliferyl alpha-D-glucopyranoside as substrate | Sus scrofa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-methylumbelliferyl-alpha-D-glucopyranoside + H2O | - |
Sus scrofa | 4-methylumbelliferone + alpha-D-glucose | - |
? | |
| Glc2Man9GlcNAc2 + 2 H2O | - |
Sus scrofa | Man9GlcNAc2 + 2 D-glucose | - |
? | |
| Glc2Man9GlcNAc2 + H2O | - |
Sus scrofa | Man9GlcNAc2 + alpha-D-glucose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 100000, SDS-PAGE | Sus scrofa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 7.5 | - |
Sus scrofa |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
inactivation below | Sus scrofa |
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