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Literature summary for 3.2.1.207 extracted from

  • Saxena, S.; Shailubhai, K.; Dong-Yu, B.; Vijay, I.K.
    Purification and characterization of glucosidase II involved in N-linked glycoprotein processing in bovine mammary gland (1987), Biochem. J., 247, 563-570.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.207

Inhibitors

Inhibitors Comment Organism Structure
Ag+
-
 Bos taurus
Hg2+
-
 Bos taurus
maltose
-
 Bos taurus
turanose
-
 Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.013
-
 4-methylumbelliferyl alpha-D-glucopyranoside
-
 Bos taurus

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
 Bos taurus
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
260000 290000 gel filtration Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Glc2Man9GlcNAc2 + H2O Bos taurus
-
 Man9GlcNAc2 + alpha-D-glucose
-
 ?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
 cow
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein N-linked oligosaccharides, high mannose type Bos taurus

Purification (Commentary)

Purification (Comment) Organism
-
 Bos taurus

Reaction

Reaction Comment Organism Reaction ID
Glc2Man9GlcNAc2-[protein] + H2O = GlcMan9GlcNAc2-[protein] + beta-D-glucopyranose Glc2Man9GlcNAc2 + H2O = Man9GlcNAc2 + glucose Bos taurus
a

Source Tissue

Source Tissue Comment Organism Textmining
mammary gland
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.096
-
 4-methylumbelliferyl alpha-D-glucopyranoside as substrate Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl alpha-D-glucopyranoside + H2O
-
 Bos taurus 4-methylumbelliferone + alpha-D-glucopyranose
-
 ?
Glc2Man9GlcNAc2 + 2 H2O
-
 Bos taurus Man9GlcNAc2 + 2 D-glucose
-
 ?
Glc2Man9GlcNAc2 + H2O
-
 Bos taurus Man9GlcNAc2 + alpha-D-glucose
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 63000-65000, SDS-PAGE, comparison with other values Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 7
-
 Bos taurus