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Literature summary for 3.2.1.203 extracted from
- Purification and characterization of an alkaline cellulase produced by Bacillus subtilis (AS3) (2013), Prep. Biochem. Biotechnol., 43, 256-270.
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 30000 | - |
x * 30000, SDS-PAGE | Bacillus subtilis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | - |
- |
- |
| Bacillus subtilis AS3 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| DEAE-Sepharose column chromatography | Bacillus subtilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (carboxymethyl)cellulose + H2O | - |
Bacillus subtilis | ? | - |
? |  |
| beta-glucan + H2O | - |
Bacillus subtilis | ? | - |
? | |
| hydroxyethylcellulose + H2O | - |
Bacillus subtilis | ? | - |
? | |
| laminarin + H2O | - |
Bacillus subtilis | ? | - |
? | |
| lichenan + H2O | - |
Bacillus subtilis | ? | - |
? | |
| additional information | the enzyme shows negligible activity with crystalline substrate such as Avicel and filter paper | Bacillus subtilis | ? | - |
? | |
| steam-exploded bagasse + H2O | - |
Bacillus subtilis | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 30000, SDS-PAGE | Bacillus subtilis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CMCase | - |
Bacillus subtilis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
- |
Bacillus subtilis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 60 | the enzyme is stable in temperature range of 20°C to 45°C and retains more than 50% activity at 60°C for 30 min | Bacillus subtilis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9.2 | - |
- |
Bacillus subtilis |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 12 | the enzyme is stable in the pH range 6-10 and retains 70% activity at pH 12.0 | Bacillus subtilis |
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