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Literature summary for 3.2.1.202 extracted from

  • Keyhani, N.; Roseman, S.
    The chitin catabolic cascade in the marine bacterium Vibrio furnissii: Molecular cloning, isolation, and characterization of a periplasmic chitodextrinase (1996), J. Biol. Chem., 271, 33414-33424.
    View publication on PubMed
Search for more literature for 3.2.1.202

Inhibitors

Inhibitors Comment Organism Structure
2-acetamido-2-deoxy-beta-D-glucopyranosyl-(1->4)-2-acetamido-2-deoxy-beta-D-glucopyranosyl-(1->4)-2-acetamido-2-deoxy-beta-D-glucopyranose
-
 Vibrio furnissii
4-nitrophenyl beta-D-N,N',N''-triacetylchitotrioside
-
 Vibrio furnissii
4-nitrophenyl beta-D-N-acetylglucosamine
-
 Vibrio furnissii
Hg2+ 5 mM, 25% loss of activity Vibrio furnissii
KCl 10-30% decrease in activity Vibrio furnissii
additional information not affected by 5 mM EDTA or EGTA, nor by the addition of up to 5mM Ca2+ or Zn2+ Vibrio furnissii
N,N',N'',N''',N'''',N'''''-hexacetylchitohexaose
-
 Vibrio furnissii
N,N',N'',N''',N''''-pentacetylchitopentaose
-
 Vibrio furnissii
N,N',N'',N'''-tetraacetylchitotetraose
-
 Vibrio furnissii
N,N'-diacetylchitobiose
-
 Vibrio furnissii
NaCl activity remains relatively constant over the range 50-250 mM NaCl (pH 7.0), but decreases significantly below and above these concentrations Vibrio furnissii

Localization

Localization Comment Organism GeneOntology No. Textmining
periplasm
-
 Vibrio furnissii
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 2 mM, 20-25% stimulation Vibrio furnissii
NaCl activity remains relatively constant over the range 50-250 mM NaCl (pH 7.0), but decreases significantly below and above these concentrations Vibrio furnissii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
112690
-
-
 Vibrio furnissii

Organism

Organism UniProt Comment Textmining
Vibrio furnissii P96156
-
-
Vibrio furnissii UniProt P96156
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification srquence contains an N-terminal 31 amino acids signal peptide Vibrio furnissii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
325
-
 substrate 4-nitrophenyl beta-D-N,N'-diacetylchitobioside, pH 7.5, 37°C Vibrio furnissii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl beta-D-N,N'-diacetylchitobioside + H2O
-
 Vibrio furnissii 4-nitrophenol + N,N'-diacetylchitobiose
-
 ?
4-nitrophenyl beta-D-N,N'-diacetylchitobioside + H2O
-
 Vibrio furnissii UniProt 4-nitrophenol + N,N'-diacetylchitobiose
-
 ?
additional information the enzyme converts chitin oligosaccharides to GlcNAc and (GlcNAc)2. No substrates: chitin, glucosamine oligosaccharides, glycoproteins, and glycopeptides containing (GlcNAc)2 Vibrio furnissii ?
-
 ?
additional information the enzyme converts chitin oligosaccharides to GlcNAc and (GlcNAc)2. No substrates: chitin, glucosamine oligosaccharides, glycoproteins, and glycopeptides containing (GlcNAc)2 Vibrio furnissii UniProt ?
-
 ?
N,N',N'',N''',N'''',N'''''-hexaacetylchitohexaose + H2O
-
 Vibrio furnissii 2 N,N',N''-triacetylchitotriose
-
 ?
N,N',N'',N''',N'''',N'''''-hexaacetylchitohexaose + H2O
-
 Vibrio furnissii UniProt 2 N,N',N''-triacetylchitotriose
-
 ?
N,N',N'',N''',N''''-pentaacetylchitopentaose + H2O
-
 Vibrio furnissii N,N'-diacetylchitobiose + N,N',N''-triacetylchitotriose
-
 ?
N,N',N'',N''',N''''-pentaacetylchitopentaose + H2O
-
 Vibrio furnissii UniProt N,N'-diacetylchitobiose + N,N',N''-triacetylchitotriose
-
 ?
N,N',N'',N'''-tetraacetylchitotetraose + H2O
-
 Vibrio furnissii 2 N,N'-diacetylchitobiose
-
 ?
N,N',N'',N'''-tetraacetylchitotetraose + H2O
-
 Vibrio furnissii UniProt 2 N,N'-diacetylchitobiose
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 112380, calculated, 1 * 120000, SDS-PAGE Vibrio furnissii

Synonyms

Synonyms Comment Organism
endoI
-
 Vibrio furnissii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35 37
-
 Vibrio furnissii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
52
-
 15 min, complete inactivation Vibrio furnissii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 7
-
 Vibrio furnissii

pH Range

pH Minimum pH Maximum Comment Organism
7.5
-
 58-70% of maximum activity Vibrio furnissii

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.077
-
 N,N',N'',N''',N'''',N'''''-hexacetylchitohexaose pH 7.5, 37°C Vibrio furnissii
0.088
-
 N,N',N'',N''',N''''-pentacetylchitopentaose pH 7.5, 37°C Vibrio furnissii
0.09
-
 N,N',N'',N'''-tetraacetylchitotetraose pH 7.5, 37°C Vibrio furnissii
0.22
-
 4-nitrophenyl beta-D-N,N',N''-triacetylchitotrioside pH 7.5, 37°C Vibrio furnissii
0.57
-
 2-acetamido-2-deoxy-beta-D-glucopyranosyl-(1->4)-2-acetamido-2-deoxy-beta-D-glucopyranosyl-(1->4)-2-acetamido-2-deoxy-beta-D-glucopyranose pH 7.5, 37°C Vibrio furnissii
0.65
-
 4-nitrophenyl beta-D-N-acetylglucosamine pH 7.5, 37°C Vibrio furnissii
1.025
-
 N,N'-diacetylchitobiose pH 7.5, 37°C Vibrio furnissii