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Literature summary for 3.2.1.201 extracted from

  • Horiuchi, A.; Aslam, M.; Kanai, T.; Atomi, H.
    A structurally novel chitinase from the chitin-degrading hyperthermophilic archaeon Thermococcus chitonophagus (2016), Appl. Environ. Microbiol., 82, 3554-3562 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression of variants in Escherichia coli, one without the signal sequence and the other corresponding only to the putative catalytic domain Thermococcus chitonophagus

Organism

Organism UniProt Comment Textmining
Thermococcus chitonophagus A0A161KIT4
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-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
colloidal chitin + H2O
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Thermococcus chitonophagus N,N'-diacetylchitobiose + N,N',N''-triacetylchitotriose
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?
additional information enzyme hydrolyzes GlcNAc oligomers longer than (GlcNAc)4. ChiD is an exo-type chitinase that releases (GlcNAc)2 or (GlcNAc)3. ChiD recognizes the reducing end of chitin chains Thermococcus chitonophagus ?
-
?
N, N', N'', N''',N'''',N'''''-hexaacetylchitohexaose + H2O
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Thermococcus chitonophagus N,N'-diacetylchitobiose + N,N',N''-triacetylchitotriose
-
?
N, N', N'', N''',N''''-pentaacetylchitopentaose + H2O
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Thermococcus chitonophagus N,N'-diactelchitobiose + N,N',N''-triacetylchitotriose
-
?
N, N', N'', N'''-tetraacetylchitotetraose + H2O
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Thermococcus chitonophagus 2 N,N'-diacetylchitobiose
-
?

Synonyms

Synonyms Comment Organism
ChiD
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Thermococcus chitonophagus

General Information

General Information Comment Organism
metabolism the presence of the two chitin-binding domains significantly enhance the termostability of the catalytic domain Thermococcus chitonophagus