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Literature summary for 3.2.1.201 extracted from

  • Papanikolau, Y.; Petratos, K.
    Application of the effects of ionic strength reducing agents in the purification and crystallization of chitinase A (2002), Acta Crystallogr. Sect. D, 58, 1593-1596.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
ChiA crystallizes both by salting-in and salting-out in the presence of a variety of precipitating agents in a wide range of concentrations and pH values Serratia marcescens

General Stability

General Stability Organism
protein precipitates at concentrations higher than 10 mg/ml in a variety of pH and ionic strength values Serratia marcescens

Organism

Organism UniProt Comment Textmining
Serratia marcescens O83008
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Purification (Commentary)

Purification (Comment) Organism
ChiA binds nonspecifically polysaccharides, which are found in the growth medium. These sugar impurities have to be removed during the purification process Serratia marcescens