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Literature summary for 3.2.1.200 extracted from

  • Gaber, Y.; Mekasha, S.; Vaaje-Kolstad, G.; Eijsink, V.G.H.; Fraaije, M.W.
    Characterization of a chitinase from the cellulolytic actinomycete Thermobifida fusca (2016), Biochim. Biophys. Acta, 1864, 1253-1259 .
    View publication on PubMed
Search for more literature for 3.2.1.200

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Thermobifida fusca

Inhibitors

Inhibitors Comment Organism Structure
colloidal chitin substrate inhibition Thermobifida fusca

Organism

Organism UniProt Comment Textmining
Thermobifida fusca Q47RL1
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography and Superdex 75 gel filtration Thermobifida fusca

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-chitin + H2O
-
 Thermobifida fusca ?
-
 ?
beta-chitin + H2O
-
 Thermobifida fusca ?
-
 ?
colloidal chitin + H2O minimal activity Thermobifida fusca ?
-
 ?
additional information no activity with N,N',N''-triacetylchitotriose Thermobifida fusca ?
-
 ?
N,N',N'',N''',N'''',N'''''-hexaacetylchitohexaose + H2O
-
 Thermobifida fusca N,N',N''-triacetylchitotriose + N,N'-diacetylchitobiose + N,N',N'',N'''-tetraacetylchitotetraose
-
 ?
N,N',N'',N''',N''''-pentaacetylchitopentaose + H2O
-
 Thermobifida fusca N,N'-diacetylchitobiose + N,N',N''-triacetylchitotriose
-
 ?
N,N',N'',N'''-tetraacetylchitotetraose + H2O
-
 Thermobifida fusca 2 N,N'-diacetylchitobiose
-
 ?

Subunits

Subunits Comment Organism
? x * 50000, SDS-PAGE Thermobifida fusca
? x * 46300, calculated from amino acid sequence Thermobifida fusca

Synonyms

Synonyms Comment Organism
GH18 chitinase
-
 Thermobifida fusca
Tfu0868
-
 Thermobifida fusca

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40 45
-
 Thermobifida fusca

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
57.5
-
 melting temperature Thermobifida fusca

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 7
-
 Thermobifida fusca

pH Range

pH Minimum pH Maximum Comment Organism
6 7.8 the enzyme displays more than 50% of maximum activity in the pH range of 6.0 to 7.8, with a peak between 6.5 and 7.0 Thermobifida fusca