Literature summary for 3.2.1.200 extracted from

Sugimoto, H.; Nakamura, K.; Nishino, Y.; Idezawa, Y.; Fujinuma, A.; Suzuki, K.; Watanabe, T.
Differences in the roles of the two surface-exposed tyrosine residues, Y240 and Y481, of Serratia marcescens chitinase B during processive degradation of crystalline chitin (2016), J. Gen. Appl. Microbiol., 61, 255-261 .

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Protein Variants

Protein Variants	Comment	Organism
Y240W	the mutant retains its substrate-binding ability but shows significantly decreased hydrolytic activity against crystalline beta-chitin compared to the wild type enzyme	Serratia marcescens
Y240W/Y481W	the mutant retains its substrate-binding ability but shows significantly decreased hydrolytic activity against crystalline beta-chitin compared to the wild type enzyme	Serratia marcescens
Y481W	the mutant retains its substrate-binding ability and shows wild type hydrolytic activity against crystalline beta-chitin	Serratia marcescens

Organism

Organism	UniProt	Comment	Textmining
Serratia marcescens	P11797	-	-
Serratia marcescens 2170	P11797	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-methylumbelliferyl beta-D-N,N'-diacetylchitobioside + H2O	-	Serratia marcescens	?	-	?
4-methylumbelliferyl beta-D-N,N'-diacetylchitobioside + H2O	-	Serratia marcescens 2170	?	-	?
crystalline beta-chitin + H2O	the enzyme degrades the chitin from the non-reducing end	Serratia marcescens	N,N'-diacetylchitobiose + ?	-	?
crystalline beta-chitin + H2O	the enzyme degrades the chitin from the non-reducing end	Serratia marcescens 2170	N,N'-diacetylchitobiose + ?	-	?
glycol chitin + H2O	-	Serratia marcescens	?	-	?
glycol chitin + H2O	-	Serratia marcescens 2170	?	-	?

Subunits

Subunits	Comment	Organism
?	x * 50000, SDS-PAGE	Serratia marcescens

Synonyms

Synonyms	Comment	Organism
ChiB	-	Serratia marcescens
chitinase B	-	Serratia marcescens

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Release 2023.1 (January 2023)