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Literature summary for 3.2.1.200 extracted from

  • Sorbotten, A.; Horn, S.J.; Eijsink, V.G.; Varum, K.M.
    Degradation of chitosans with chitinase B from Serratia marcescens. Production of chito-oligosaccharides and insight into enzyme processivity (2005), FEBS J., 272, 538-549.
    View publication on PubMed

Organism

Organism UniProt Comment Textmining
Serratia marcescens Q54276
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chitosan + H2O
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Serratia marcescens ? 65% acetylated chitosan, enzyme shows a processive mode of action, moving the sugar chain two residues at a time and almost exclusively yielding oligomers with even-numbered chain lengths ?
additional information ChiB is an exoenzyme which degrades the polymer chains from their nonreducing ends. The degradation of chitosan with 65% acetylated units showed biphasic kinetics: the faster phase is dominated by cleavage on the reducing side of two acetylated units (occupying subsites -2 and -1), while the slower kinetic phase reflects cleavage on the reducing side of a deacetylated and an acetylated unit (bound to subsites -2 and -1, respectively). The enzyme does not show preferences with respect to acetylation of the sugar bound in the +1 subsite. Even chitosans with low degrees of acetylation can be degraded by ChiB Serratia marcescens ?
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Synonyms

Synonyms Comment Organism
ChiB
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Serratia marcescens