Literature summary for 3.2.1.200 extracted from

Ohnuma, T.; Numata, T.; Osawa, T.; Mizuhara, M.; Lampela, O.; Juffer, A.H.; Skriver, K.; Fukamizo, T.
A class V chitinase from Arabidopsis thaliana: gene responses, enzymatic properties, and crystallographic analysis (2011), Planta, 234, 123-137.

Search for more literature for 3.2.1.200

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Arabidopsis thaliana

Crystallization (Commentary)

Crystallization (Comment)	Organism
to 2.0 A resolution, and docking simulation with substrate N,N',N'',N''',N''''-pentaacetylchitopentaose	Arabidopsis thaliana

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
30000	-	-	Arabidopsis thaliana

Organism

Organism	UniProt	Comment	Textmining
Arabidopsis thaliana	O81862	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
proteolytic modification	protein contains a signal sequence consisting of 24 amino acid residues at the N-terminus	Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	the enzyme hydrolyzes N-acetylglucosamine oligomers producing dimers from the nonreducing end of the substrates. It shows predominantly exo-splitting mode, but contains a slight endo-splitting activity and catalyzes an anomeric retention reaction. The enzyme catalyzes transglycosylation reaction in a low efficiency	Arabidopsis thaliana	?	-	?
N,N',N'',N''',N'''',N'''''-hexaacetylchitohexaose + H2O	-	Arabidopsis thaliana	N,N'-diacetylchitobiose + N,N',N'',N'''-tetraacetylchitotetraose	-	?
N,N',N'',N''',N''''-pentaacetylchitopentaose + H2O	-	Arabidopsis thaliana	N,N'-diacetylchitobiose + N,N',N''-triacetylchitotriose	-	?
N,N',N'',N'''-tetraacetylchitotetraose + H2O	-	Arabidopsis thaliana	2 N,N'-diacetylchitobiose	-	?

Subunits

Subunits	Comment	Organism
?	x * 39031, calculated, x * 30000, SDS-PAGE, mature protein	Arabidopsis thaliana

Synonyms

Synonyms	Comment	Organism
At4g19810	-	Arabidopsis thaliana
ChiC	-	Arabidopsis thaliana

Expression

Organism	Comment	Expression
Arabidopsis thaliana	expression is induced by both biotic and abiotic stress	up

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)