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Literature summary for 3.2.1.20 extracted from

  • Jung, J.H.; Seo, D.H.; Holden, J.F.; Kim, H.S.; Baik, M.Y.; Park, C.S.
    Broad substrate specificity of a hyperthermophilic alpha-glucosidase from Pyrobaculum arsenaticum (2016), Food Sci. Biotechnol., 25, 1665-1669 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.20

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pyrobaculum arsenaticum

Organism

Organism UniProt Comment Textmining
Pyrobaculum arsenaticum A4WMH3
-
-
Pyrobaculum arsenaticum DSM 13514 A4WMH3
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.84
-
 substrate maltose, pH 5.0, 90°C Pyrobaculum arsenaticum
5.84
-
 substrate nigerose, pH 5.0, 90°C Pyrobaculum arsenaticum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
maltose + H2O
-
 Pyrobaculum arsenaticum 2 D-glucose
-
 ?
maltose + H2O
-
 Pyrobaculum arsenaticum DSM 13514 2 D-glucose
-
 ?
maltotriose + 2 H2O 44% of the activity with maltose Pyrobaculum arsenaticum 3 D-glucose
-
 ?
maltotriose + 2 H2O 44% of the activity with maltose Pyrobaculum arsenaticum DSM 13514 3 D-glucose
-
 ?
additional information the enzyme mainly recognizes the non-reducing end of the substrate, releasing the glucose unit. Multifunctional enzyme that catalyzes the hydrolysis of terminal alpha-1,2-linked, alpha-1,3-linked and alpha-1,4-linked glucose residues, e.g. reaction of kojibiose hydrolase Pyrobaculum arsenaticum ?
-
 ?
additional information the enzyme mainly recognizes the non-reducing end of the substrate, releasing the glucose unit. Multifunctional enzyme that catalyzes the hydrolysis of terminal alpha-1,2-linked, alpha-1,3-linked and alpha-1,4-linked glucose residues, e.g. reaction of kojibiose hydrolase Pyrobaculum arsenaticum DSM 13514 ?
-
 ?
nigerose + H2O
-
 Pyrobaculum arsenaticum 2 D-glucose
-
 ?
nigerose + H2O
-
 Pyrobaculum arsenaticum DSM 13514 2 D-glucose
-
 ?

Synonyms

Synonyms Comment Organism
Pars_2044
-
 Pyrobaculum arsenaticum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
90
-
-
 Pyrobaculum arsenaticum

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
80
-
 more than 70% of maximum activity Pyrobaculum arsenaticum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Pyrobaculum arsenaticum

pH Range

pH Minimum pH Maximum Comment Organism
4 6 more than 90% of maximum activity Pyrobaculum arsenaticum