Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | - |
Thermoascus aurantiacus | |
Cr3+ | - |
Thermoascus aurantiacus | |
Hg2+ | - |
Thermoascus aurantiacus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | the metal ions Na+, Ba2+, Co2+, Ni2+, Mg2+, Mn2+, Al3+, Zn2+, Ca2+ do not or poorly influence the enzyme activity | Thermoascus aurantiacus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
83000 | - |
x * 83000, SDS-PAGE | Thermoascus aurantiacus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermoascus aurantiacus | - |
- |
- |
Thermoascus aurantiacus CBMAI 756 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme 172fold by ultrafiltration, ammonium sulfate fractionation, anion exchange chromatograpyh, and two different steps of gel filtration | Thermoascus aurantiacus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
404 | - |
purified enzyme, pH 4.5, 70°C | Thermoascus aurantiacus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
maltoheptaose + H2O | - |
Thermoascus aurantiacus | ? | - |
? | |
maltoheptaose + H2O | - |
Thermoascus aurantiacus CBMAI 756 | ? | - |
? | |
maltohexaose + H2O | - |
Thermoascus aurantiacus | ? | - |
? | |
maltohexaose + H2O | - |
Thermoascus aurantiacus CBMAI 756 | ? | - |
? | |
maltopentaose + H2O | - |
Thermoascus aurantiacus | ? | - |
? | |
maltopentaose + H2O | - |
Thermoascus aurantiacus CBMAI 756 | ? | - |
? | |
maltose + H2O | - |
Thermoascus aurantiacus | alpha-D-glucose + D-glucose | - |
? | |
maltotetraose + H2O | - |
Thermoascus aurantiacus | ? | - |
? | |
maltotetraose + H2O | - |
Thermoascus aurantiacus CBMAI 756 | ? | - |
? | |
maltotriose + H2O | - |
Thermoascus aurantiacus | ? | - |
? | |
additional information | the enzyme shows a transglycosylation property, by the release of oligosaccharides after 3 h of incubation with maltose, and specificity for short maltooligosaccharides, the enzymatic activity decreases following an increase in the polymerization degree of short maltooligosaccharides, low activity with maltoheptaose, no activity with starch | Thermoascus aurantiacus | ? | - |
? | |
additional information | the enzyme shows a transglycosylation property, by the release of oligosaccharides after 3 h of incubation with maltose, and specificity for short maltooligosaccharides, the enzymatic activity decreases following an increase in the polymerization degree of short maltooligosaccharides, low activity with maltoheptaose, no activity with starch | Thermoascus aurantiacus CBMAI 756 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 83000, SDS-PAGE | Thermoascus aurantiacus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
- |
Thermoascus aurantiacus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 80 | activity range, profile, overview | Thermoascus aurantiacus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 60 | purified enzyme, 60% activity remaining | Thermoascus aurantiacus |
70 | - |
purified enzyme, 30% activity remaining | Thermoascus aurantiacus |
80 | - |
purified enzyme, no activity remaining | Thermoascus aurantiacus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
- |
Thermoascus aurantiacus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
2.5 | 7 | activity range, profile, overview | Thermoascus aurantiacus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | 9 | purified enzyme, completely stable | Thermoascus aurantiacus |