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Literature summary for 3.2.1.20 extracted from

  • Carvalho, A.F.; Boscolo, M.; da Silva, R.; Ferreira, H.; Gomes, E.
    Purification and characterization of the alpha-glucosidase produced by thermophilic fungus Thermoascus aurantiacus CBMAI 756 (2010), J. Microbiol., 48, 452-459.
    View publication on PubMed
Search for more literature for 3.2.1.20

Inhibitors

Inhibitors Comment Organism Structure
Ag+
-
 Thermoascus aurantiacus
Cr3+
-
 Thermoascus aurantiacus
Hg2+
-
 Thermoascus aurantiacus

Metals/Ions

Metals/Ions Comment Organism Structure
additional information the metal ions Na+, Ba2+, Co2+, Ni2+, Mg2+, Mn2+, Al3+, Zn2+, Ca2+ do not or poorly influence the enzyme activity Thermoascus aurantiacus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
83000
-
 x * 83000, SDS-PAGE Thermoascus aurantiacus

Organism

Organism UniProt Comment Textmining
Thermoascus aurantiacus
-
-
-
Thermoascus aurantiacus CBMAI 756
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 172fold by ultrafiltration, ammonium sulfate fractionation, anion exchange chromatograpyh, and two different steps of gel filtration Thermoascus aurantiacus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
404
-
 purified enzyme, pH 4.5, 70°C Thermoascus aurantiacus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
maltoheptaose + H2O
-
 Thermoascus aurantiacus ?
-
 ?
maltoheptaose + H2O
-
 Thermoascus aurantiacus CBMAI 756 ?
-
 ?
maltohexaose + H2O
-
 Thermoascus aurantiacus ?
-
 ?
maltohexaose + H2O
-
 Thermoascus aurantiacus CBMAI 756 ?
-
 ?
maltopentaose + H2O
-
 Thermoascus aurantiacus ?
-
 ?
maltopentaose + H2O
-
 Thermoascus aurantiacus CBMAI 756 ?
-
 ?
maltose + H2O
-
 Thermoascus aurantiacus alpha-D-glucose + D-glucose
-
 ?
maltotetraose + H2O
-
 Thermoascus aurantiacus ?
-
 ?
maltotetraose + H2O
-
 Thermoascus aurantiacus CBMAI 756 ?
-
 ?
maltotriose + H2O
-
 Thermoascus aurantiacus ?
-
 ?
additional information the enzyme shows a transglycosylation property, by the release of oligosaccharides after 3 h of incubation with maltose, and specificity for short maltooligosaccharides, the enzymatic activity decreases following an increase in the polymerization degree of short maltooligosaccharides, low activity with maltoheptaose, no activity with starch Thermoascus aurantiacus ?
-
 ?
additional information the enzyme shows a transglycosylation property, by the release of oligosaccharides after 3 h of incubation with maltose, and specificity for short maltooligosaccharides, the enzymatic activity decreases following an increase in the polymerization degree of short maltooligosaccharides, low activity with maltoheptaose, no activity with starch Thermoascus aurantiacus CBMAI 756 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 83000, SDS-PAGE Thermoascus aurantiacus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
-
 Thermoascus aurantiacus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
50 80 activity range, profile, overview Thermoascus aurantiacus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40 60 purified enzyme, 60% activity remaining Thermoascus aurantiacus
70
-
 purified enzyme, 30% activity remaining Thermoascus aurantiacus
80
-
 purified enzyme, no activity remaining Thermoascus aurantiacus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
-
 Thermoascus aurantiacus

pH Range

pH Minimum pH Maximum Comment Organism
2.5 7 activity range, profile, overview Thermoascus aurantiacus

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 9 purified enzyme, completely stable Thermoascus aurantiacus