Any feedback?
Literature summary for 3.2.1.20 extracted from
- Disaccharidase activities in camel small intestine: Biochemical investigations of maltase-glucoamylase activity (2007), Comp. Biochem. Physiol. B, 146B, 124-130.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | no inhibition of isozyme MG4, 22.5 inhibition of isozyme MG6 | Camelus dromedarius |  |
| 4-chloromercuribenzoate | 95% inhibition of isozyme MG4, no inhibition of isozyme MG6 | Camelus dromedarius | |
| 4-hydroxymercuribenzoate | 86% inhibition of isozyme MG4, 22% inhibition of isozyme MG6 | Camelus dromedarius | |
| DTNB | 66% inhibition of isozyme MG4, 41% inhibition of isozyme MG6 | Camelus dromedarius | |
| Hg2+ | - |
Camelus dromedarius | |
| iodoacetamide | 13% inhibition of isozyme MG4, 9% inhibition of isozyme MG6 | Camelus dromedarius | |
| NEM | 79% inhibition of isozyme MG4, no inhibition of isozyme MG6 | Camelus dromedarius | |
| PMSF | no inhibition of isozyme MG4, 47% inhibition of isozyme MG6 | Camelus dromedarius | |
| sucrose | inhibits the maltase activity of the enzyme | Camelus dromedarius | |
| Trypsin inhibitor | 17% inhibition of isozyme MG4, no inhibition of isozyme MG6 | Camelus dromedarius |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 13.3 | - |
maltose | pH 5.5, 37°C, isozyme MG4 | Camelus dromedarius | |
| 20 | - |
maltose | pH 5.5, 37°C, isozyme MG6 | Camelus dromedarius | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | only marginal effects of Ca2+, Mg2+, Mn2+, Co2+, and Fe3+ on the enzyme | Camelus dromedarius |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 140000 | - |
isozyme MG4, gel filtration | Camelus dromedarius |
| 160000 | - |
isozyme MG6, gel filtration | Camelus dromedarius |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Camelus dromedarius | enzymic digestion profile determination for camel small intestine, overview | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Camelus dromedarius | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native isozymes from small intestine by anion exchange chromatography and gel filtration, isozyme MG4 9fold, isozyme MG6 24fold | Camelus dromedarius |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| small intestine | isozymes MG4 and MG6 | Camelus dromedarius | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.024 | - |
glucoamylase activity with substrate starch, small intestine | Camelus dromedarius |
| 0.043 | - |
maltase activity with substrate maltose, small intestine | Camelus dromedarius |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha-cyclodextrin + H2O | low activity with isozymes MG4 and MG6 | Camelus dromedarius | alpha-D-glucose | - |
? | |
| amylopectin + H2O | - |
Camelus dromedarius | alpha-D-glucose | - |
? | |
| glycogen + H2O | - |
Camelus dromedarius | alpha-D-glucose | - |
? | |
| maltose + H2O | preferred substrate of isozymes MG4 and MG6 | Camelus dromedarius | alpha-D-glucose + D-glucose | - |
? | |
| additional information | enzymic digestion profile determination for camel small intestine, overview | Camelus dromedarius | ? | - |
? | |
| additional information | poor activity with sucrose, dextrine, lactose, and no activity with xylose | Camelus dromedarius | ? | - |
? | |
| starch + H2O | - |
Camelus dromedarius | alpha-D-glucose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 140000, isozyme MG4, SDS-PAGE, 1 * 160000, isozyme MG6, SDS-PAGE | Camelus dromedarius |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| maltase-glucoamylase | - |
Camelus dromedarius |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
isozyme MG6 | Camelus dromedarius |
| 50 | - |
isozyme MG4 | Camelus dromedarius |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
isozyme MG6, stable up to | Camelus dromedarius |
| 50 | - |
isozyme MG4, stable up to, strong decrease in activity of isozyme MG6 | Camelus dromedarius |
| 60 | - |
strong decrease in activity of isozyme MG4 | Camelus dromedarius |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
isozymes MG4 and MG6 | Camelus dromedarius |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
