Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Hg2+ | at least 80% inhibition at 5 mM | Panicum miliaceum | |
Pb2+ | at least 80% inhibition at 5 mM | Panicum miliaceum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | the KM value decreases with the increase in molecular weight of substrates | Panicum miliaceum | |
0.0013 | - |
amylopectin | pH 4.5, 37°C | Panicum miliaceum | |
0.28 | - |
amylose | pH 4.5, 37°C | Panicum miliaceum | |
0.34 | - |
kojibiose | pH 4.5, 37°C | Panicum miliaceum | |
0.39 | - |
maltoheptaose | pH 4.5, 37°C | Panicum miliaceum | |
0.45 | - |
maltohexaose | pH 4.5, 37°C | Panicum miliaceum | |
0.86 | - |
maltotetraose | pH 4.5, 37°C | Panicum miliaceum | |
1.13 | - |
maltotriose | pH 4.5, 37°C | Panicum miliaceum | |
1.46 | - |
maltose | pH 4.5, 37°C | Panicum miliaceum | |
1.46 | - |
nigerose | pH 4.5, 37°C | Panicum miliaceum | |
3.9 | - |
phenyl alpha-glucoside | pH 4.5, 37°C | Panicum miliaceum | |
9.76 | - |
isomaltose | pH 4.5, 37°C | Panicum miliaceum | |
3640 | - |
soluble starch | pH 4.5, 37°C | Panicum miliaceum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme is not affected by 5 mM of Na+, K+, Ca2+, Co2+, Mn2+, Ni2+, Mg2+, Sr2+, or Al3+ | Panicum miliaceum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
80000 | - |
gel filtration | Panicum miliaceum |
86000 | - |
1 * 86000, SDS-PAGE | Panicum miliaceum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-1,4-glucan + H2O | Panicum miliaceum | - |
alpha-D-glucose | - |
? | |
amylopectin + H2O | Panicum miliaceum | in starch, preferred substrate | alpha-D-glucose | - |
? | |
malto-oligosaccharides + H2O | Panicum miliaceum | - |
alpha-D-glucose | - |
? | |
maltoheptaose + 6 H2O | Panicum miliaceum | - |
7 alpha-D-glucose | - |
? | |
maltose + H2O | Panicum miliaceum | - |
alpha-D-glucose + D-glucose | - |
? | |
additional information | Panicum miliaceum | the enzyme prefers partially degraded starch substrate from germinating millet seeds | ? | - |
? | |
nigerose + H2O | Panicum miliaceum | alpha-1,3-linkage, exists as abnormal linkage in starch | 2 alpha-D-glucose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Panicum miliaceum | - |
alpha-glucosidase II in germinating seeds | - |
Purification (Comment) | Organism |
---|---|
native enzyme from germinating millet seeds to homogeneity by ammonium sulfate fractionation, cation exchange chromatography, gel filtration, hydrophobic interaction chromatography and preparative isoelectric focusing | Panicum miliaceum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seed | germinating, alpha-glucosidase II | Panicum miliaceum | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Panicum miliaceum |
76 | - |
purified native enzyme | Panicum miliaceum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-1,4-glucan + H2O | - |
Panicum miliaceum | alpha-D-glucose | - |
? | |
amylopectin + H2O | preferred substrate | Panicum miliaceum | alpha-D-glucose | - |
? | |
amylopectin + H2O | in starch, preferred substrate | Panicum miliaceum | alpha-D-glucose | - |
? | |
amylose + H2O | amylose EX-1 | Panicum miliaceum | alpha-D-glucose | - |
? | |
isomaltose + H2O | very low activity | Panicum miliaceum | 2 alpha-D-glucose | - |
? | |
kojibiose + H2O | - |
Panicum miliaceum | alpha-D-glucose + D-glucose | - |
? | |
malto-oligosaccharides + H2O | - |
Panicum miliaceum | alpha-D-glucose | - |
? | |
maltoheptaose + 6 H2O | - |
Panicum miliaceum | 7 alpha-D-glucose | - |
? | |
maltohexaose + 5 H2O | - |
Panicum miliaceum | 6 alpha-D-glucose | - |
? | |
maltose + H2O | - |
Panicum miliaceum | alpha-D-glucose + D-glucose | - |
? | |
maltotetraose + 3 H2O | - |
Panicum miliaceum | 4 alpha-D-glucose | - |
? | |
maltotriose + 2 H2O | - |
Panicum miliaceum | 3 alpha-D-glucose | - |
? | |
additional information | the enzyme prefers partially degraded starch substrate from germinating millet seeds | Panicum miliaceum | ? | - |
? | |
additional information | the enzyme hydrolyzes polymer substrate more rapidly than maltose, substrate specificity, activity with insoluble substrates, overview | Panicum miliaceum | ? | - |
? | |
nigerose + H2O | alpha-1,3-linkage, exists as abnormal linkage in starch | Panicum miliaceum | 2 alpha-D-glucose | - |
? | |
phenyl alpha-glucoside + H2O | low activity | Panicum miliaceum | alpha-D-glucose + phenol | - |
? | |
soluble starch + H2O | very low activity | Panicum miliaceum | alpha-D-glucose | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 86000, SDS-PAGE | Panicum miliaceum |
Synonyms | Comment | Organism |
---|---|---|
alpha-glucosidase II | - |
Panicum miliaceum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 60 | - |
Panicum miliaceum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
purified native enzyme, stable up to | Panicum miliaceum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | 1.97 | kojibiose | pH 4.5, 37°C | Panicum miliaceum | |
3.8 | - |
phenyl alpha-glucoside | pH 4.5, 37°C | Panicum miliaceum | |
5.75 | - |
isomaltose | pH 4.5, 37°C | Panicum miliaceum | |
9.44 | - |
kojibiose | pH 4.5, 37°C | Panicum miliaceum | |
24.2 | - |
amylopectin | pH 4.5, 37°C | Panicum miliaceum | |
26.2 | - |
amylose | pH 4.5, 37°C | Panicum miliaceum | |
33.5 | - |
maltose | pH 4.5, 37°C | Panicum miliaceum | |
35.7 | - |
nigerose | pH 4.5, 37°C | Panicum miliaceum | |
36.1 | - |
maltohexaose | pH 4.5, 37°C | Panicum miliaceum | |
40.7 | - |
maltotetraose | pH 4.5, 37°C | Panicum miliaceum | |
43.9 | - |
maltotriose | pH 4.5, 37°C | Panicum miliaceum | |
47.5 | - |
maltoheptaose | pH 4.5, 37°C | Panicum miliaceum | |
49.7 | - |
soluble starch | pH 4.5, 37°C | Panicum miliaceum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | 5 | - |
Panicum miliaceum |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
2.5 | 8 | purified native enzyme, stable | Panicum miliaceum |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Panicum miliaceum | isoelectric focusing | - |
8.3 |