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Literature summary for 3.2.1.20 extracted from

  • Yamasaki, Y.; Fujimoto, M.; Kariya, J.; Konno, H.
    Purification and characterization of an alpha-glucosidase from germinating millet seeds (2005), Phytochemistry, 66, 851-857.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Hg2+ at least 80% inhibition at 5 mM Panicum miliaceum
Pb2+ at least 80% inhibition at 5 mM Panicum miliaceum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information the KM value decreases with the increase in molecular weight of substrates Panicum miliaceum
0.0013
-
amylopectin pH 4.5, 37°C Panicum miliaceum
0.28
-
amylose pH 4.5, 37°C Panicum miliaceum
0.34
-
kojibiose pH 4.5, 37°C Panicum miliaceum
0.39
-
maltoheptaose pH 4.5, 37°C Panicum miliaceum
0.45
-
maltohexaose pH 4.5, 37°C Panicum miliaceum
0.86
-
maltotetraose pH 4.5, 37°C Panicum miliaceum
1.13
-
maltotriose pH 4.5, 37°C Panicum miliaceum
1.46
-
maltose pH 4.5, 37°C Panicum miliaceum
1.46
-
nigerose pH 4.5, 37°C Panicum miliaceum
3.9
-
phenyl alpha-glucoside pH 4.5, 37°C Panicum miliaceum
9.76
-
isomaltose pH 4.5, 37°C Panicum miliaceum
3640
-
soluble starch pH 4.5, 37°C Panicum miliaceum

Metals/Ions

Metals/Ions Comment Organism Structure
additional information the enzyme is not affected by 5 mM of Na+, K+, Ca2+, Co2+, Mn2+, Ni2+, Mg2+, Sr2+, or Al3+ Panicum miliaceum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
80000
-
gel filtration Panicum miliaceum
86000
-
1 * 86000, SDS-PAGE Panicum miliaceum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
alpha-1,4-glucan + H2O Panicum miliaceum
-
alpha-D-glucose
-
?
amylopectin + H2O Panicum miliaceum in starch, preferred substrate alpha-D-glucose
-
?
malto-oligosaccharides + H2O Panicum miliaceum
-
alpha-D-glucose
-
?
maltoheptaose + 6 H2O Panicum miliaceum
-
7 alpha-D-glucose
-
?
maltose + H2O Panicum miliaceum
-
alpha-D-glucose + D-glucose
-
?
additional information Panicum miliaceum the enzyme prefers partially degraded starch substrate from germinating millet seeds ?
-
?
nigerose + H2O Panicum miliaceum alpha-1,3-linkage, exists as abnormal linkage in starch 2 alpha-D-glucose
-
?

Organism

Organism UniProt Comment Textmining
Panicum miliaceum
-
alpha-glucosidase II in germinating seeds
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme from germinating millet seeds to homogeneity by ammonium sulfate fractionation, cation exchange chromatography, gel filtration, hydrophobic interaction chromatography and preparative isoelectric focusing Panicum miliaceum

Source Tissue

Source Tissue Comment Organism Textmining
seed germinating, alpha-glucosidase II Panicum miliaceum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Panicum miliaceum
76
-
purified native enzyme Panicum miliaceum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-1,4-glucan + H2O
-
Panicum miliaceum alpha-D-glucose
-
?
amylopectin + H2O preferred substrate Panicum miliaceum alpha-D-glucose
-
?
amylopectin + H2O in starch, preferred substrate Panicum miliaceum alpha-D-glucose
-
?
amylose + H2O amylose EX-1 Panicum miliaceum alpha-D-glucose
-
?
isomaltose + H2O very low activity Panicum miliaceum 2 alpha-D-glucose
-
?
kojibiose + H2O
-
Panicum miliaceum alpha-D-glucose + D-glucose
-
?
malto-oligosaccharides + H2O
-
Panicum miliaceum alpha-D-glucose
-
?
maltoheptaose + 6 H2O
-
Panicum miliaceum 7 alpha-D-glucose
-
?
maltohexaose + 5 H2O
-
Panicum miliaceum 6 alpha-D-glucose
-
?
maltose + H2O
-
Panicum miliaceum alpha-D-glucose + D-glucose
-
?
maltotetraose + 3 H2O
-
Panicum miliaceum 4 alpha-D-glucose
-
?
maltotriose + 2 H2O
-
Panicum miliaceum 3 alpha-D-glucose
-
?
additional information the enzyme prefers partially degraded starch substrate from germinating millet seeds Panicum miliaceum ?
-
?
additional information the enzyme hydrolyzes polymer substrate more rapidly than maltose, substrate specificity, activity with insoluble substrates, overview Panicum miliaceum ?
-
?
nigerose + H2O alpha-1,3-linkage, exists as abnormal linkage in starch Panicum miliaceum 2 alpha-D-glucose
-
?
phenyl alpha-glucoside + H2O low activity Panicum miliaceum alpha-D-glucose + phenol
-
?
soluble starch + H2O very low activity Panicum miliaceum alpha-D-glucose
-
?

Subunits

Subunits Comment Organism
monomer 1 * 86000, SDS-PAGE Panicum miliaceum

Synonyms

Synonyms Comment Organism
alpha-glucosidase II
-
Panicum miliaceum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50 60
-
Panicum miliaceum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
purified native enzyme, stable up to Panicum miliaceum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.04 1.97 kojibiose pH 4.5, 37°C Panicum miliaceum
3.8
-
phenyl alpha-glucoside pH 4.5, 37°C Panicum miliaceum
5.75
-
isomaltose pH 4.5, 37°C Panicum miliaceum
9.44
-
kojibiose pH 4.5, 37°C Panicum miliaceum
24.2
-
amylopectin pH 4.5, 37°C Panicum miliaceum
26.2
-
amylose pH 4.5, 37°C Panicum miliaceum
33.5
-
maltose pH 4.5, 37°C Panicum miliaceum
35.7
-
nigerose pH 4.5, 37°C Panicum miliaceum
36.1
-
maltohexaose pH 4.5, 37°C Panicum miliaceum
40.7
-
maltotetraose pH 4.5, 37°C Panicum miliaceum
43.9
-
maltotriose pH 4.5, 37°C Panicum miliaceum
47.5
-
maltoheptaose pH 4.5, 37°C Panicum miliaceum
49.7
-
soluble starch pH 4.5, 37°C Panicum miliaceum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5 5
-
Panicum miliaceum

pH Stability

pH Stability pH Stability Maximum Comment Organism
2.5 8 purified native enzyme, stable Panicum miliaceum

pI Value

Organism Comment pI Value Maximum pI Value
Panicum miliaceum isoelectric focusing
-
8.3