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Literature summary for 3.2.1.20 extracted from

  • Ogawa, M.; Nishio, T.; Minoura, K.; Uozumi, T.; Wada, M.; Hashimoto, N.; Kawachi, R.; Oku, T.
    Recombinant alpha-glucosidase from Aspergillus niger. Overexpression by Emericella nidulans, purification and characterization (2006), J. Appl. Glycosci., 53, 13-16.
No PubMed abstract available
Search for more literature for 3.2.1.20

Cloned(Commentary)

Cloned (Comment) Organism
gene aglA, overexpression of the extracellular enzyme in Emericella nidulans strain JCM10259 Aspergillus niger

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.59
-
 4-nitrophenyl alpha-D-glucopyranoside pH 4.5, 37°C, recombinant enzyme Aspergillus niger
0.62
-
 4-nitrophenyl alpha-D-glucopyranoside pH 4.5, 37°C, wild-type enzyme Aspergillus niger
1.04
-
 maltose pH 4.5, 37°C, recombinant enzyme Aspergillus niger
1.25
-
 maltose pH 4.5, 37°C, wild-type enzyme Aspergillus niger
6.54
-
 4-Nitrophenyl 2-deoxy-alpha-D-glucopyranoside pH 4.5, 37°C, wild-type enzyme Aspergillus niger
6.61
-
 4-Nitrophenyl 2-deoxy-alpha-D-glucopyranoside pH 4.5, 37°C, recombinant enzyme Aspergillus niger
10.5
-
 4-nitrophenyl 3-deoxy-alpha-D-glucopyranoside pH 4.5, 37°C, wild-type enzyme Aspergillus niger
11.6
-
 4-nitrophenyl 3-deoxy-alpha-D-glucopyranoside pH 4.5, 37°C, recombinant enzyme Aspergillus niger

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33000
-
 1 * 98000 + 1 * 33000, recombinant enzyme, SDS-PAGE Aspergillus niger
98000
-
 1 * 98000 + 1 * 33000, recombinant enzyme, SDS-PAGE Aspergillus niger
120000
-
 recombinant enzyme, native PAGE Aspergillus niger
125000
-
 wild-type enzyme, native PAGE Aspergillus niger

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
maltose + H2O Aspergillus niger
-
 alpha-D-glucose + D-glucose
-
 ?
maltose + H2O Aspergillus niger GN-3
-
 alpha-D-glucose + D-glucose
-
 ?

Organism

Organism UniProt Comment Textmining
Aspergillus niger P56526 gene aglA
-
Aspergillus niger GN-3 P56526 gene aglA
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein the wild-type enzyme contains over 35% more sugar than the recombinant enzyme Aspergillus niger

Purification (Commentary)

Purification (Comment) Organism
native enzyme and recombinant secreted extracellular enzyme from Emericella nidulans strain JCM10259 by ammonium sulfate fractionation, anion exchange chromatography, and gel filtration to homogeneity Aspergillus niger

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.13
-
 purified recombinant enzyme, substrate 4-nitrophenyl 3-deoxy-alpha-D-glucopyranoside, recombinant enzyme Aspergillus niger
0.14
-
 purified wild-type enzyme, substrate 4-nitrophenyl 3-deoxy-alpha-D-glucopyranoside, recombinant enzyme Aspergillus niger
0.96
-
 purified recombinant enzyme, substrate 4-nitrophenyl alpha-D-glucopyranoside, recombinant enzyme Aspergillus niger
1.06
-
 purified wild-type enzyme, substrate 4-nitrophenyl alpha-D-glucopyranoside, recombinant enzyme Aspergillus niger
3.7
-
 purified recombinant enzyme, substrate 4-nitrophenyl 2-deoxy-alpha-D-glucopyranoside, recombinant enzyme Aspergillus niger
4.25
-
 purified wild-type enzyme, substrate 4-nitrophenyl 2-deoxy-alpha-D-glucopyranoside, recombinant enzyme Aspergillus niger
46.8
-
 purified recombinant enzyme, substrate maltose, recombinant enzyme Aspergillus niger
57.8
-
 purified wild-type enzyme, substrate maltose, recombinant enzyme Aspergillus niger

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl 2-deoxy-alpha-D-glucopyranoside + H2O low activity, wild-type and recombinant enzyme Aspergillus niger 4-nitrophenol + 2-deoxy-alpha-D-glucose
-
 ?
4-nitrophenyl 2-deoxy-alpha-D-glucopyranoside + H2O low activity, wild-type and recombinant enzyme Aspergillus niger GN-3 4-nitrophenol + 2-deoxy-alpha-D-glucose
-
 ?
4-nitrophenyl 3-deoxy-alpha-D-glucopyranoside + H2O very low activity, wild-type and recombinant enzyme Aspergillus niger 4-nitrophenol + 3-deoxy-alpha-D-glucose
-
 ?
4-nitrophenyl alpha-D-glucopyranoside + H2O very low activity, wild-type and recombinant enzyme Aspergillus niger 4-nitrophenol + alpha-D-glucose
-
 ?
4-nitrophenyl alpha-D-glucopyranoside + H2O very low activity, wild-type and recombinant enzyme Aspergillus niger GN-3 4-nitrophenol + alpha-D-glucose
-
 ?
maltose + H2O
-
 Aspergillus niger alpha-D-glucose + D-glucose
-
 ?
maltose + H2O preferred substrate of wild-type and recombinant enzyme Aspergillus niger alpha-D-glucose + D-glucose
-
 ?
maltose + H2O
-
 Aspergillus niger GN-3 alpha-D-glucose + D-glucose
-
 ?
maltose + H2O preferred substrate of wild-type and recombinant enzyme Aspergillus niger GN-3 alpha-D-glucose + D-glucose
-
 ?
additional information no activity of wild-type and recombinant enzyme with 4-nitrophenyl 4-deoxy-alpha-D-glucopyranoside and 4-nitrophenyl 6-deoxy-alpha-D-glucopyranoside Aspergillus niger ?
-
 ?
additional information no activity of wild-type and recombinant enzyme with 4-nitrophenyl 4-deoxy-alpha-D-glucopyranoside and 4-nitrophenyl 6-deoxy-alpha-D-glucopyranoside Aspergillus niger GN-3 ?
-
 ?

Subunits

Subunits Comment Organism
dimer 1 * 98000 + 1 * 33000, recombinant enzyme, SDS-PAGE Aspergillus niger

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 wild-type and recombinant enzyme Aspergillus niger

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
 wild-type and recombinant enzyme Aspergillus niger