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Literature summary for 3.2.1.20 extracted from
- Copy-DNA cloning and characterisation of a potato alpha-glucosidase: expression in Escherichia coli and effects of down-regulation in transgenic potato (2001), Planta, 213, 258-264.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene MAL2, genomic library construction, DNA and amino acid sequence determination and analysis, overexpression in Escherichia coli as N- and C-terminally His-tagged protein, expression pattern analysis of the gene in potato plants by Northern blot analysis | Solanum tuberosum |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | construction of transgenic potato plants, transformation of the antisense construct via Agrobacterium tumefaciens infection, gene is cloned under the control of CaMV S35 promotor, the gene is down-regulated in transgenic plants showing no obvious phenotype, but showing reduced content of sucrose and increased content of fructose and glucose in tubers | Solanum tuberosum |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3.7 | - |
maltotetraose | pH 6.8, 30°C, recombinant enzyme | Solanum tuberosum |  |
| 4.7 | - |
maltopentaose | pH 6.8, 30°C, recombinant enzyme | Solanum tuberosum | |
| 6.9 | - |
maltotriose | pH 6.8, 30°C, recombinant enzyme | Solanum tuberosum | |
| 8.1 | - |
4-nitrophenyl alpha-D-glucopyranoside | pH 6.8, 30°C, recombinant enzyme | Solanum tuberosum | |
| 15.1 | - |
maltoheptaose | pH 6.8, 30°C, recombinant enzyme | Solanum tuberosum | |
| 33 | - |
maltohexaose | pH 6.8, 30°C, recombinant enzyme | Solanum tuberosum | |
| 34.5 | - |
maltose | pH 6.8, 30°C, recombinant enzyme | Solanum tuberosum | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Solanum tuberosum | phylogenetic analysis | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Solanum tuberosum | - |
gene MAL2w, enzyme MAL2 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from Escherichia coli by metal affinity chromatography | Solanum tuberosum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Solanum tuberosum | - |
| additional information | expression pattern analysis | Solanum tuberosum | - |
| tuber | - |
Solanum tuberosum | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl alpha-D-glucopyranoside + H2O | - |
Solanum tuberosum | 4-nitrophenol + alpha-D-glucopyranose | - |
? | |
| maltoheptaose + H2O | - |
Solanum tuberosum | maltohexaose + D-glucose | - |
? | |
| maltohexaose + H2O | - |
Solanum tuberosum | maltopentaose + D-glucose | - |
? | |
| maltopentaose + H2O | - |
Solanum tuberosum | D-glucose + ? | - |
? | |
| maltose + H2O | - |
Solanum tuberosum | 2 D-glucose | - |
? | |
| maltotetraose + H2O | - |
Solanum tuberosum | maltotriose + D-glucose | - |
? | |
| maltotriose + H2O | - |
Solanum tuberosum | maltose + D-glucose | - |
? | |
| additional information | phylogenetic analysis | Solanum tuberosum | ? | - |
? | |
| additional information | no activity with xyloglucan oligosaccharides, 4-nitrophenyl alpha-D-xylopyranoside, and gelatinised potato starch | Solanum tuberosum | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| MAL2 | - |
Solanum tuberosum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Solanum tuberosum |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 35.2 | - |
maltoheptaose | pH 6.8, 30°C, recombinant enzyme | Solanum tuberosum | |
| 35.9 | - |
maltose | pH 6.8, 30°C, recombinant enzyme | Solanum tuberosum | |
| 57.2 | - |
maltohexaose | pH 6.8, 30°C, recombinant enzyme | Solanum tuberosum | |
| 64.3 | - |
maltopentaose | pH 6.8, 30°C, recombinant enzyme | Solanum tuberosum | |
| 69.6 | - |
4-nitrophenyl alpha-D-glucopyranoside | pH 6.8, 30°C, recombinant enzyme | Solanum tuberosum | |
| 74.6 | - |
maltotetraose | pH 6.8, 30°C, recombinant enzyme | Solanum tuberosum | |
| 100.4 | - |
maltotriose | pH 6.8, 30°C, recombinant enzyme | Solanum tuberosum | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | - |
assay at | Solanum tuberosum |
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