Cloned (Comment) | Organism |
---|---|
gene MAL2, genomic library construction, DNA and amino acid sequence determination and analysis, overexpression in Escherichia coli as N- and C-terminally His-tagged protein, expression pattern analysis of the gene in potato plants by Northern blot analysis | Solanum tuberosum |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of transgenic potato plants, transformation of the antisense construct via Agrobacterium tumefaciens infection, gene is cloned under the control of CaMV S35 promotor, the gene is down-regulated in transgenic plants showing no obvious phenotype, but showing reduced content of sucrose and increased content of fructose and glucose in tubers | Solanum tuberosum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.7 | - |
maltotetraose | pH 6.8, 30°C, recombinant enzyme | Solanum tuberosum | |
4.7 | - |
maltopentaose | pH 6.8, 30°C, recombinant enzyme | Solanum tuberosum | |
6.9 | - |
maltotriose | pH 6.8, 30°C, recombinant enzyme | Solanum tuberosum | |
8.1 | - |
4-nitrophenyl alpha-D-glucopyranoside | pH 6.8, 30°C, recombinant enzyme | Solanum tuberosum | |
15.1 | - |
maltoheptaose | pH 6.8, 30°C, recombinant enzyme | Solanum tuberosum | |
33 | - |
maltohexaose | pH 6.8, 30°C, recombinant enzyme | Solanum tuberosum | |
34.5 | - |
maltose | pH 6.8, 30°C, recombinant enzyme | Solanum tuberosum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Solanum tuberosum | phylogenetic analysis | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Solanum tuberosum | - |
gene MAL2w, enzyme MAL2 | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli by metal affinity chromatography | Solanum tuberosum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Solanum tuberosum | - |
additional information | expression pattern analysis | Solanum tuberosum | - |
tuber | - |
Solanum tuberosum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl alpha-D-glucopyranoside + H2O | - |
Solanum tuberosum | 4-nitrophenol + alpha-D-glucopyranose | - |
? | |
maltoheptaose + H2O | - |
Solanum tuberosum | maltohexaose + D-glucose | - |
? | |
maltohexaose + H2O | - |
Solanum tuberosum | maltopentaose + D-glucose | - |
? | |
maltopentaose + H2O | - |
Solanum tuberosum | D-glucose + ? | - |
? | |
maltose + H2O | - |
Solanum tuberosum | 2 D-glucose | - |
? | |
maltotetraose + H2O | - |
Solanum tuberosum | maltotriose + D-glucose | - |
? | |
maltotriose + H2O | - |
Solanum tuberosum | maltose + D-glucose | - |
? | |
additional information | phylogenetic analysis | Solanum tuberosum | ? | - |
? | |
additional information | no activity with xyloglucan oligosaccharides, 4-nitrophenyl alpha-D-xylopyranoside, and gelatinised potato starch | Solanum tuberosum | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MAL2 | - |
Solanum tuberosum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Solanum tuberosum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
35.2 | - |
maltoheptaose | pH 6.8, 30°C, recombinant enzyme | Solanum tuberosum | |
35.9 | - |
maltose | pH 6.8, 30°C, recombinant enzyme | Solanum tuberosum | |
57.2 | - |
maltohexaose | pH 6.8, 30°C, recombinant enzyme | Solanum tuberosum | |
64.3 | - |
maltopentaose | pH 6.8, 30°C, recombinant enzyme | Solanum tuberosum | |
69.6 | - |
4-nitrophenyl alpha-D-glucopyranoside | pH 6.8, 30°C, recombinant enzyme | Solanum tuberosum | |
74.6 | - |
maltotetraose | pH 6.8, 30°C, recombinant enzyme | Solanum tuberosum | |
100.4 | - |
maltotriose | pH 6.8, 30°C, recombinant enzyme | Solanum tuberosum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | - |
assay at | Solanum tuberosum |