Application | Comment | Organism |
---|---|---|
nutrition | usage in industrial processes to hydrolyze starch, saccharification step | Pyrococcus furiosus |
Cloned (Comment) | Organism |
---|---|
functional overexpression in Escherichia coli | Pyrococcus furiosus |
General Stability | Organism |
---|---|
DTT and urea stabilize against thermal inactivation | Pyrococcus furiosus |
guanidine hydrochloride inactivates | Pyrococcus furiosus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | - |
Pyrococcus furiosus | |
guanidine hydrochloride | inactivation | Pyrococcus furiosus | |
SDS | partial inactivation | Pyrococcus furiosus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | thermal dependence of kinetics, overview | Pyrococcus furiosus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Thermococcus sp. | - |
- |
intracellular | - |
Saccharolobus solfataricus | 5622 | - |
intracellular | - |
Pyrococcus woesei | 5622 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no requirement for Ca2+ | Pyrococcus furiosus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
1 * 60000 | Thermococcus sp. |
80000 | - |
x * 80000 | Saccharolobus solfataricus |
125000 | - |
- |
Pyrococcus furiosus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
maltose + H2O | Pyrococcus furiosus | - |
2 D-glucose | - |
? | |
additional information | Pyrococcus furiosus | the enzyme catalyzes the final step in the metabolism of carbohydrates, such as starch, to glucose for cell utilization, the oligosaccharide transport system shows high affinity for trehalose and maltose | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus furiosus | - |
strain DSM 3638 | - |
Pyrococcus woesei | - |
- |
- |
Saccharolobus solfataricus | - |
- |
- |
Saccharolobus solfataricus 98/2 | - |
- |
- |
Thermococcus sp. | - |
- |
- |
Thermococcus sp. AN1 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Thermococcus sp. |
2 different protocols, 5-step procedure including ammonium sulfate precipitation and several chromatographic steps, 310fold | Pyrococcus furiosus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | continuous culture, growth on yeast extract, tryptone and starch | Pyrococcus furiosus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
185 | - |
purified enzyme, substrate methyl alpha-D-glucopyranoside | Pyrococcus furiosus |
287 | - |
purified enzyme, substrate 4-nitrophenyl alpha-D-glucopyranoside | Pyrococcus furiosus |
951 | - |
purified enzyme, substrate isomaltose | Pyrococcus furiosus |
1760 | - |
purified enzyme, substrate maltose | Pyrococcus furiosus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl alpha-D-glucopyranoside + H2O | - |
Pyrococcus furiosus | 4-nitrophenol + alpha-D-glucopyranose | - |
? | |
4-nitrophenyl alpha-D-glucopyranoside + H2O | preferred substrate | Thermococcus sp. | 4-nitrophenol + alpha-D-glucopyranose | - |
? | |
4-nitrophenyl alpha-D-glucopyranoside + H2O | preferred substrate | Thermococcus sp. AN1 | 4-nitrophenol + alpha-D-glucopyranose | - |
? | |
isomaltose + H2O | - |
Pyrococcus furiosus | 2 D-glucose | - |
? | |
maltopentaose + H2O | preferred substrate | Pyrococcus woesei | D-glucose + ? | - |
? | |
maltose + H2O | - |
Pyrococcus furiosus | 2 D-glucose | - |
? | |
maltose + H2O | best substrate | Pyrococcus furiosus | 2 D-glucose | - |
? | |
maltose + H2O | preferred substrate | Saccharolobus solfataricus | 2 D-glucose | - |
? | |
maltose + H2O | preferred substrate | Saccharolobus solfataricus 98/2 | 2 D-glucose | - |
? | |
methyl alpha-D-glucopyranoside + H2O | - |
Pyrococcus furiosus | methanol + alpha-D-glucopyranose | - |
? | |
additional information | the enzyme catalyzes the final step in the metabolism of carbohydrates, such as starch, to glucose for cell utilization, the oligosaccharide transport system shows high affinity for trehalose and maltose | Pyrococcus furiosus | ? | - |
? | |
additional information | sucrose is a poor or no substrate, no activity with starch | Pyrococcus furiosus | ? | - |
? | |
oligosaccharides + H2O | various substrates, enzyme is exo-acting to release alpha-D-glucose, preferential cleavage of the alpha-1,4-bond | Pyrococcus furiosus | alpha-D-glucose | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 55000-60000, SDS-PAGE | Pyrococcus furiosus |
monomer | 1 * 60000 | Thermococcus sp. |
tetramer or pentamer | x * 80000 | Saccharolobus solfataricus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
98 | - |
at pH 5.0-6.0 | Pyrococcus furiosus |
105 | - |
- |
Saccharolobus solfataricus |
105 | - |
- |
Pyrococcus woesei |
105 | 115 | at pH 5.5 | Pyrococcus furiosus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
95 | - |
purified enzyme, stable 3 h | Thermococcus sp. |
98 | - |
half-life 30 min | Thermococcus sp. |
98 | - |
half-life 160-180 min | Saccharolobus solfataricus |
98 | - |
pH 6.5, loss of 50% activity within 48 h, in presence of 0.1 M DTT or 1 M urea 80% activity are retained for 30 min, enzyme shows loss of 78% activity in presence of 1 M SDS within 30 min, and loss of 50% activity in presence of 20 mM EDTA, complete inactivation occurs in presence of 1 M guanidine hydrochloride within 30 min | Pyrococcus furiosus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
- |
Saccharolobus solfataricus |
5 | 5.5 | - |
Pyrococcus woesei |
5.5 | - |
assay at | Pyrococcus furiosus |
7.5 | - |
- |
Thermococcus sp. |