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Literature summary for 3.2.1.20 extracted from

  • Nishimoto, M.; Kubota, M.; Tsuji, M.; Mori, H.; Kimura, A.; Matsui, H.; Chiba, S.
    Purification and substrate specificity of honeybee, Apis mellifera L., alpha-glucosidase III (2001), Biosci. Biotechnol. Biochem., 65, 1610-1616.
    View publication on PubMed
Search for more literature for 3.2.1.20

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information determination of subsite affinities, kinetics Apis mellifera
2 3 phenyl alpha-D-glucoside pH 5.5, 35°C Apis mellifera
10
-
 maltotriose pH 5.5, 35°C Apis mellifera
11
-
 maltose pH 5.5, 35°C Apis mellifera
13
-
 4-nitrophenyl alpha-D-glucopyranoside pH 5.5, 35°C Apis mellifera
16
-
 maltotetraose pH 5.5, 35°C Apis mellifera
30
-
 sucrose pH 5.5, 35°C Apis mellifera
31
-
 turanose pH 5.5, 35°C Apis mellifera
44
-
 nigerose pH 5.5, 35°C Apis mellifera
56
-
 isomaltose pH 5.5, 35°C Apis mellifera
63
-
 maltopentaose pH 5.5, 35°C Apis mellifera
63
-
 maltohexaose pH 5.5, 35°C Apis mellifera
67
-
 kojibiose pH 5.5, 35°C Apis mellifera

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
68000
-
 about, gel filtration Apis mellifera

Organism

Organism UniProt Comment Textmining
Apis mellifera
-
 alpha-glucosidase III
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein about 7.4% carbohydrate content Apis mellifera

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, by salting-out chromatography and 5 additional chromatographic steps Apis mellifera

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Apis mellifera
11.3
-
 purified enzyme Apis mellifera

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl alpha-D-glucopyranoside + H2O high activity Apis mellifera 4-nitrophenol + alpha-D-glucopyranose
-
 ?
isomaltose + H2O low activity Apis mellifera 2 alpha-D-glucose
-
 ?
kojibiose + H2O
-
 Apis mellifera alpha-D-glucose + D-glucose
-
 ?
maltohexaose + H2O low activity Apis mellifera maltopentaose + D-glucose
-
 ?
maltopentaose + H2O
-
 Apis mellifera alpha-D-glucose + ?
-
 ?
maltose + H2O
-
 Apis mellifera alpha-D-glucose + D-glucose
-
 ?
maltotetraose + H2O
-
 Apis mellifera alpha-D-glucose
-
 ?
maltotriose + H2O best substrate Apis mellifera maltose + alpha-D-glucose
-
 ?
additional information maltooligosachharides of four or more units of polymerization are slowly hydrolyzed Apis mellifera ?
-
 ?
nigerose + H2O
-
 Apis mellifera 2 alpha-D-glucose
-
 ?
phenyl alpha-D-glucoside + H2O
-
 Apis mellifera phenol + alpha-D-glucose
-
 ?
phenyl maltoside + H2O
-
 Apis mellifera phenol + alpha-D-glucose
-
 ?
sucrose + H2O
-
 Apis mellifera alpha-D-glucose + beta-D-fructose
-
 ?
turanose + H2O
-
 Apis mellifera alpha-D-glucose + D-fructose
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 68000, SDS-PAGE Apis mellifera

Synonyms

Synonyms Comment Organism
alpha-glucosidase III
-
 Apis mellifera
HBG III
-
 Apis mellifera

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 stable up to Apis mellifera
60
-
 15 min, complete inactivation Apis mellifera

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Apis mellifera

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
 Apis mellifera

pH Stability

pH Stability pH Stability Maximum Comment Organism
4.5
-
 labile below pH 4.5 Apis mellifera
5 10 stable Apis mellifera